ADAMTS proteases : methods and protocols

This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, __ADAMTS Proteases: Methods and Protocols__ is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature. Front Matter ....Pages i-x ADAMTS Proteins: Concepts, Challenges, and Prospects (Suneel S. Apte)....Pages 1-12 Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology (Carlos Peris-Torres, Orlando Serrano, María del Carmen Plaza-Calonge, Juan Carlos Rodríguez-Manzaneque)....Pages 13-24 Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays (Steven John Berardinelli, Robert S. Haltiwanger)....Pages 25-43 Challenges and Solutions for Purification of ADAMTS Proteases: An Overview (Alain C. Colige)....Pages 45-53 Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay (Alain C. Colige)....Pages 55-62 ADAMTS7: Recombinant Protein Expression and Purification (Rens de Groot)....Pages 63-73 Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants (Milan M. Fowkes, Ngee H. Lim)....Pages 75-91 Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins (Takao Kohno, Himari Ogino, Yuko Yamakage, Mitsuharu Hattori)....Pages 93-104 Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain (Himari Ogino, Yuko Yamakage, Mihoshi B. Yamashita, Takao Kohno, Mitsuharu Hattori)....Pages 105-111 Chemical Modification of Proteoglycanases with Biotin (Salvatore Santamaria)....Pages 113-123 Analysis of Aggrecanase Activity Using Neoepitope Antibodies (Salvatore Santamaria, Kazuhiro Yamamoto)....Pages 125-136 Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4 (Mukti Singh, Stuart A. Cain, Clair Baldock)....Pages 137-155 Purification of Recombinant ADAMTSL2 (Mukti Singh, Clair Baldock)....Pages 157-172 Expression Analysis by RNAscopeTM In Situ Hybridization (Timothy J. Mead, Suneel S. Apte)....Pages 173-178 Analysis of ADAMTS Effects on Cell Adhesion and Migration (Jordi Lambert, Dylan R. Edwards)....Pages 179-193 Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils (Dirk Hubmacher)....Pages 195-206 Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton (Timothy J. Mead)....Pages 207-212 Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins (Valentin Nelea, Dieter P. Reinhardt)....Pages 213-235 Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins (Rong-Mo Zhang, Heena Kumra, Dieter P. Reinhardt)....Pages 237-250 Visualization of Perineuronal Nets in Central Nervous System Tissue Sections (Luke Souter, Jessica C. F. Kwok)....Pages 251-260 Visualization and Quantification of Pericellular Matrix (Timothy J. Mead, Suneel S. Apte)....Pages 261-264 Quantitative Mass Spectrometry-Based Secretome Analysis as a Tool to Investigate Metalloprotease and TIMP Activity (Chun-Yao Yang, Linda Troeberg, Simone D. Scilabra)....Pages 265-273 Preparation and Application of a Decellularized Extracellular Matrix for Identification of ADAMTS Substrates (Rahel Schnellmann, Ruth Chiquet-Ehrismann)....Pages 275-284 Mapping the N-Terminome in Tissue Biopsies by PCT-TAILS (Louise Bundgaard, Simonas Savickas, Ulrich auf dem Keller)....Pages 285-296 Back Matter ....Pages 297-299 "This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature. ." - Prové de l'editor