Antibody Techniques

The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Key Features * Detailed, easy-to-follow, step-by-step protocols * Convenient, easy-to-use format * Extensive practical information * Essential background information * Helpful hints Content: Front Matter , Page iii Copyright , Page iv CONTRIBUTORS , Pages xiii-xiv PREFACE , Pages xv-xvi , ERIK P. LILLEHOJ, VEDPAL S. MALIK 1 - ANTIBODY MOLECULES AND THE IMMUNE RESPONSE: THEORETICAL BACKGROUND , Pages 1-48 , IAN M. ZITRON 2 - ADJUVANT-FREE POLYCLONAL ANTIBODY RESPONSE MANIPULATED BY ANTIBODY- MEDIATED ANTIGEN TARGETING , Pages 49-69 , DENIS P. SNIDER 3 - HYBRIDOMAS AND MONOCLONAL ANTIBODIES , Pages 71-102 , CATHY HEFFNER BARRETT 4 - PCR AMPLIFICATION OF ANTIBODY GENES , Pages 103-114 , JAMES W. LARRICK, ROBERT F. BALINT 5 - LABORATORY ANIMAL CARE AND USE , Pages 115-139 , PATRICIA C. ALLEN 6 - ADJUVANTS , Pages 141-151 , ANTHONY C. ALLISON, NOELENE E. BYARS 7 - LARGE-SCALE PRODUCTION OF MONOCLONAL ANTIBODIES , Pages 153-175 , CRISTINA GLAD, INGE NILSSON 8 - ANTIBODY-BINDING BACTERIAL PROTEINS AS IMMUNOREAGENTS , Pages 177-209 , MICHAEL D.P. BOYLE, DENNIS W. METZGER 9 - CONJUGATING ANTIBODIES TO LIGANDS, ENZYMES, AND METALLIC PARTICLES , Pages 211-225 , BYUNG-KIL CHOE 10 - DOT IMMUNOBINDING (DIB), ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), AND RADIOIMMUNOASSAY (RIA) FOR DETECTING PEPTIDE ANTIGENS AND SPECIFIC ANTIBODIES , Pages 227-258 , EDGAR LAURITZEN, HANNE FLYGE, ARNE HOLM 11 - IMMUNOSTAINING CELLS AND TISSUES , Pages 259-272 , BYUNG-KIL CHOE, YONGOCK CHO 12 - PROTEIN IMMUNOBLOTTING , Pages 273-289 , ERIK P. LILLEHOJ 13 - FLOW CYTOMETRY AND FLUORESCENCE-ACTIVATED CELL SORTING , Pages 291-305 , HYUN S. LILLEHOJ, ALISON MARTIN 14 - SCREENING EXPRESSION LIBRARIES WITH ANTIBODIES , Pages 307-326 , WILLIAM J. PATRIE 15 - IMMUNOAFFINITY PURIFICATION , Pages 327-341 , JOHN THARAKAN INDEX , Pages 343-353 The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research.
This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage.

Key Features
* Detailed, easy-to-follow, step-by-step protocols
* Convenient, easy-to-use format
* Extensive practical information
* Essential background information
* Helpful hints The applicability of immunotechniques to a variety of research problems in many areas of biology and chemistry has expanded. This book presents specific antibody molecules which provide means of separation, quantitative and qualitative analysis, and localization It is useful to anyone doing biological or biochemical research.