Cell-cell interactions : methods and protocols

In the second edition of __Cell-Cell Interactions: Methods and Protocols,__ expert researchers in the field detail a collection of protocols to examine interactions between cells. These include protocols that focus on both __in vivo__ and __in vitro__ methods-based approaches. New updated chapters also bring together many currently used assays in examining cell-cell interactions and the biological consequences of those interactions. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and Practical, __Cell-Cell Interactions: Methods and Protocol, Second Edition__ is a valuable resource for researchers who are already involved in the cell-cell interaction field, and also for those that are new to the area. Proteomics analysis of contact-initiated eph receptor-ephrin signaling / Claus Jorgensen and Alexei Poliakov Control of vascular tube morphogenesis and maturation in 3d extracellular matrices by endothelial cells and pericytes / George E. Davis, Dae Joong Kim, Chun-Xia Meng, Pieter R. Norden, Katherine R. Speichinger, Matthew T. Davis, Annie O. Smith, Stephanie L.K. Bowers and Amber N. Stratman Analyzing cell-cell interactions in 3-dimensional adhesion assays / Stephanie L.K. Bowers and Troy A. Baudino Production of spontaneously beating neonatal at heart tissue for calcium and contractile studies / Fnu Gerilechaogetu, Hao Feng, Honey B. Golden, Damir Nizamutdiov, Donald M. Foster, Shannon Glaser and David E. Dostal Paracrine communication between mechanically stretched myocytes and fibroblasts / Hao Feng, Fnu Gerilechaogetu, Honey B. Golden, Damir Nizamutdinov, Donald M. Foster, Shannon Glaser and David E. Dostal Assessing blood-brain barrier function using In vitro assays / Joseph Bressler, Katherine Clark, and Cliona O'Driscoll Methods to assess tissue permeability / Juan C. Ibla and Joseph Khoury In vivo quantification of metastatic tumor cell adhesion in the pulmonary microvasculature / F. Bartsch, M.L. Kang, S.T. Mees, J. Haier and P. Gassmann Cell membrane vesicles as a tool for the study of direct eithelial-stromal interaction: lessons from CD147 / Eric Gabison, Farah Khayati, Samia Mourah and Suzanne Menashi Microencapsulation of stem cells to study cellular interactions / Keith Moore, Adam Vandergriff, and Jay D. Potts Cell-surface protein-protein interaction analysis with time-resolved FRET and snap-tag technologies / Timothy N. Feinstein Single cell analysis of lipid rafts / William T. Lee Micropatterning cell adhesion on polyacrylamide hydrogels / Jian Zhang, Wei-hui Guo, Andrew Rape and Yu-li Wang Measuring cell-cell tugging forces using bowtie- patterned mPADs (microarray post detectors) / Daniel M. Cohen, Mike T. Yang, and Christopher S. Chen Generation and analysis of biosensors to measure mechanical forces within cells / Katharina Austen, Carleen Kluger, Andrea Freikamp, Anna Chrostek-Grashoff, and Carsten Grashoff Proteomic analysis of the left ventricle post-myocardial infarction to identify in vivo candidate matrix metalloproteinase substrates / Andriy Yabluchanskiy, Yaojun Li, Lisandra E. de Castro Brás, Kevin Hakala, Susan T. Weintraub and Merry L. Lindsey. Front Matter....Pages i-xii Back Matter....Pages 1-16 ....Pages 17-28