Cell Cycle - Materials and Methods (Springer Lab Manuals)

During their lifetime, especially when growing and dividing, cells go through various steps of the cell cycle. Knowledge of the individual steps of the cell cycle will help us understand the development of a variety of diseases better, including cancer, and also to design new drugs against it. New techniques for studying the molecular basis of these processes have recently been developed and are described in detail in this manual. A glossary helps the reader to cope with the complex cell cycle terminology. An Historical Synopsis Of Cell Cycle Terms / M. Isabel Chiu And Michele Pagano -- Use Of Immunohistochemistry In Cell Cycle Studies / Jirina Bartkova, Jiri Lukas, And Jiri Bartek -- Immunofluorescence Techniques In Cell Cycle Studies / M. Cristina Cardoso And Heinrich Leonhardt -- Fluorescence In Situ Hybridization And Human Cell Cycle Genes / Douglas J. Demetrick -- Techniques For Monitoring Cell Cycle Phases / Marina Giunta And Carlo Pucillo -- Centrifugal Elutriation / Robert L. Marraccino And Peter C. Keng -- Synchronization Of Mammalian Cells / Patrick M. O'connor And Joany Jackman -- Microinjection And Electroporation Of Macromolecules Into Live Cells / Rainer Pepperkok -- Stable And Transient Transfections In Cell Cycle Studies / Mark Rolfe -- Quantification Of Premature And Normal Mitosis / Sun W. Tam And Robert Schlegel -- Preparation Of Xenopus Egg Extracts And Their Utilization In Cell Cycle Studies / Paul R. Clarke --^ Cloning And Expression Of Cell Cycle Genes In Schizosaccharomyces Pombe / Guillame Cottarel -- Preparation Of Synchronous Cultures Of The Fission Yeast, Schizosaccharomyces Pombe / James Creanor -- Identification Of Novel Cell Cycle Targets Using Small Molecule Ligands / Vivian Berlin And M. Isabel Chiu -- Bacterial Expression And In Vitro Assay Of Cell Cycle Kinases / Lisa Connell-crowley And Wade Harper -- Heterologous Expression And Secretion Of Cell Cycle Proteins Using The Yeast Pichia Pastoris / Guillaume Cottarel -- Sv 40 Based In Vitro Dna Replication Assay / Anindya Dutta And Ellen Winchester -- From Sequence To Function-structural Motifs Of Cell Cycle Proteins / Jens W. Eckstein -- Purification Of Cell Cycle Kinases / Didier Fesquet, Jean-claude Labbé, And Jean-claude Cavadore -- Interaction-based Cloning Of Novel Proteins / Francesca Fiore, Lou Lamphere, And Jeno Gyuris -- Expression Of Cell Cycle Proteins Using Recombinant Baculoviruses / Gregory J. Hannon --^ Cell Cycle-regulated Transcription Factors : Bandshift Assays / Pidder Jansen-dürr -- Immunoprecipitation And Immumoblotting In Cell Cycle Studies / Christopher W. Jenkins And Yue Xiong -- Protein Mapping In Cell Cycle Studies / Emma Lees -- From Peptide To Purified Antibody / Michele Pagano. Includes Bibliographical References And Index. Front Matter....Pages I-XXX Front Matter....Pages 1-1 Use of Immunohistochemistry in Cell Cycle Studies....Pages 3-14 Immunofluorescence Techniques in Cell Cycle Studies....Pages 15-28 Fluorescence in Situ Hybridization and Human Cell Cycle Genes....Pages 29-45 Techniques for Monitoring Cell Cycle Phases....Pages 46-52 Centrifugal Elutriation....Pages 53-62 Synchronization of Mammalian Cells....Pages 63-74 Microinjection and Electroporation of Macromolecules into Live Cells....Pages 75-86 Stable and Transient Transfections in Cell Cycle Studies....Pages 87-92 Quantification of Premature and Normal Mitosis....Pages 93-99 Front Matter....Pages 101-101 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies....Pages 103-116 Front Matter....Pages 117-117 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe ....Pages 119-131 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces pombe ....Pages 132-142 Front Matter....Pages 143-143 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands....Pages 145-156 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases....Pages 157-168 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Picchia pastoris ....Pages 169-174 SV40-Based in Vitro DNA Replication Assay....Pages 175-185 From Sequence to Function — Structural Motifs of Cell Cycle Proteins....Pages 186-199 Purification of Cell Cycle Kinases....Pages 200-210 Interaction-Based Cloning of Novel Proteins....Pages 211-230 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses....Pages 231-242 Front Matter....Pages 143-143 Cell Cycle-Regulated Transcription Factors; Bandshift Assays....Pages 243-249 Immunoprecipitation and Immunoblotting in Cell Cycle Studies....Pages 250-263 Protein Mapping in Cell Cycle Studies....Pages 264-270 From Peptide to Purified-Antibody....Pages 271-280 Back Matter....Pages 281-286