Developmental toxicology : methods and protocols

This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitroapproaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful __Methods in Molecular Biology__ series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, __Developmental Toxicology: Methods and Protocols, Second Edition__ serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures. Front Matter ....Pages i-xi In Vitro Models in Developmental Toxicology (Louise M. Winn)....Pages 1-6 Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments (Jason M. Hansen, Ted B. Piorczynski)....Pages 7-17 Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology (Charles A. Easley)....Pages 19-34 Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model (Mika Suga, Miho K. Furue)....Pages 35-48 Micromass Methods for the Evaluation of Developmental Toxicants (Omar J. Mohammed, Margaret K. Pratten)....Pages 49-72 The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System (France-Hélène Paradis, Han Yan, Chunwei Huang, Barbara F. Hales)....Pages 73-91 Embryonic Midfacial Palatal Organ Culture Methods in Developmental Toxicology (Barbara D. Abbott)....Pages 93-105 Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology (Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, Sakhila K. Banu)....Pages 107-127 Hepatotoxicity in Zebrafish Larvae (Kathryn Bambino, Joshua Morrison, Jaime Chu)....Pages 129-138 Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model (Joslynn G. Affleck, Virginia K. Walker)....Pages 139-153 The Chicken Embryo as a Model in Developmental Toxicology (Michael R. Stark, Micah M. Ross)....Pages 155-171 The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity (Cecilia Berg)....Pages 173-186 Mouse Whole Embryo Culture (Emily W. Y. Tung, Louise M. Winn)....Pages 187-194 Rat Whole Embryo Culture (Craig Harris)....Pages 195-217 Rabbit Whole Embryo Culture (Terence R. S. Ozolinš)....Pages 219-233 Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology (Brendon K. Warner, Jonathan K. Alder, Arminda Suli)....Pages 235-250 Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish (Jonathon T. Hill)....Pages 251-259 Assessment of Histiotrophic Nutrition Using Fluorescent Probes (Craig Harris)....Pages 261-279 Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization (Jeffery R. Barrow)....Pages 281-295 Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos (Robert M. Zucker, John M. Rogers)....Pages 297-311 Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection (Danielle M. Drake, Aaron M. Shapiro, Peter G. Wells)....Pages 313-328 Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA (Shama Bhatia, Peter G. Wells)....Pages 329-349 Western Analysis of Breast Cancer 1 Protein (BRCA1) (Danielle M. Drake, Peter G. Wells)....Pages 351-374 Regulation and Control of AP-1 Binding Activity in Embryotoxicity (Terence R. S. Ozolinš)....Pages 375-388 Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR) (Shama Bhatia, Jason Matthews, Peter G. Wells)....Pages 389-403 The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity (Rebecca D. Maciver, Terence R. S. Ozolinš)....Pages 405-420 Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining (Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, Michelle Graf)....Pages 421-434 Back Matter ....Pages 435-436 This thorough edition explores numerous methodological approaches that can be extremely informative for our understanding of the developmental toxicant mechanisms, characterization of developmental outcomes, and development of potential interventions that are clinically relevant. Presenting both staple and state-of-the-art approaches, the book focuses on numerous cellular models (induced pluripotent stem cells, neural crest culture, etc.), some less frequently used but important animal models (chick, zebrafish), in vitro approaches using whole embryos (rat, mouse and rabbit), and specific outcome methodologies to assess changes on the morphological to molecular level. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that allows for smooth processes in the lab. Authoritative and practical, Developmental Toxicology: Methods and Protocols, Second Edition serves as an ideal aid to support further investigation into the specific mechanisms and outcomes of developmental exposures.