Digital Microscopy, 3rd Edition

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition* Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods Content: Microscope Basics Review Article Pages 1-10 Greenfield Sluder, Joshua J. Nordberg The Optics of Microscope Image Formation Review Article Pages 11-42 David E. Wolf Proper Alignment of the Microscope Review Article Pages 43-53 H. Ernst Keller Mating Cameras to Microscopes Review Article Pages 55-61 Jan Hinsch Fundamentals of Fluorescence and Fluorescence Microscopy Review Article Pages 63-91 David E. Wolf Fluorescent Protein Applications in Microscopy Review Article Pages 93-113 Aaron F. Straight Live‐Cell Fluorescence Imaging Review Article Pages 115-140 Jennifer C. Waters Working with Classic Video Review Article Pages 141-157 Edward H. Hinchcliffe, Greenfield Sluder Practical Aspects of Adjusting Digital Cameras Review Article Pages 159-169 Joshua J. Nordberg, Greenfield Sluder Cameras for Digital Microscopy Review Article Pages 171-186 Kenneth R. Spring A High‐Resolution Multimode Digital Microscope System Review Article Pages 187-218 E.D. Salmon, Sidney L. Shaw, Jennifer C. Waters, Clare M. Waterman‐Storer, Paul S. Maddox, Elaine Yeh, Kerry Bloom Electronic Cameras for Low‐Light Microscopy Review Article Pages 219-249 Ivan Rasnik, Todd French, Ken Jacobson, Keith Berland Camera Technologies for Low Light Imaging: Overview and Relative Advantages Review Article Pages 251-283 Butch Moomaw Digital Manipulation of Brightfield and Fluorescence Images: Noise Reduction, Contrast Enhancement, and Feature Extraction Review Article Pages 285-314 Richard A. Cardullo, Edward H. Hinchcliffe Digital Image Files in Light Microscopy Review Article Pages 315-333 Thomas M. Durcan, Edward H. Hinchcliffe High‐Resolution Video‐Enhanced Differential Interference Contrast Light Microscopy Review Article Pages 335-364 E.D. Salmon, Phong Tran Quantitative Analysis of Digital Microscope Images Review Article Pages 365-396 David E. Wolf, Champika Samarasekera, Jason R. Swedlow Evaluating Optical Aberration Using Fluorescent Microspheres: Methods, Analysis, and Corrective Actions Review Article Pages 397-413 Paul C. Goodwin Ratio Imaging: Practical Considerations for Measuring Intracellular Ca 2+ and pH in Living Cells Review Article Pages 415-433 Nathan O'Connor, Randi B. Silver Computational Restoration of Fluorescence Images: Noise Reduction, Deconvolution, and Pattern Recognition Review Article Pages 435-445 Yu‐li Wang Quantitative Fluorescence Microscopy and Image Deconvolution Review Article Pages 447-465 Jason R. Swedlow Practical Aspects of Quantitative Confocal Microscopy Review Article Pages 467-478 John M. Murray Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy Review Article Pages 479-494 Richard A. Cardullo Fluorescence Lifetime Imaging Microscopy Review Article Pages 495-524 Ching‐Wei Chang, Dhruv Sud, Mary‐Ann Mycek Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells Review Article Pages 525-559 Dylan A. Bulseco, David E. Wolf Breaking the Resolution Limit in Light Microscopy Review Article Pages 561-580 Rainer Heintzmann, Gabriella Ficz Series Editors Page II Contents Pages V-XII Contributors to Volume 81 Pages XIII-XIV Preface Pages XV-XVII Greenfield Sluder, David E. Wolf Index Pages 581-600 Volumes in Series Pages 601-608 The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.

* Expands coverage to include discussion of confocal microscopy not found in the previous edition
* Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods