Over the past two decades, expressed sequence tags (ESTs - single pass reads from randomly selected cDNAs), have proven to be a remarkably cost-effective route for the purposes of gene discovery. Gaining in popularity, millions of ESTs have now been generated for over a thousand different species. In __Expressed Sequence Tags (ESTs): Generation and Analysis__, leading experts in the field introduce the reader to many of the fundamental concepts underlying the generation and analysis of ESTs through readily accessible and affordable sequencing technologies. The volume focuses on various methods used to generate, process and analyze EST datasets, while also exploring the use of EST technology for other purposes such as expression profiling, analysis of alternative transcripts, and phylogenomics. Written in the highly successful __Methods in Molecular BiologyTM__ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and programs, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, __Expressed Sequence Tags (ESTs): Generation and Analysis__ serves as an ideal reference for scientists continuing the vital investigation of genes and the genome. Expressed Sequence Tags : An Overview / John Parkinson And Mark Blaxter --strategies For Undertaking Expressed Sequence Tag (est) Projects / Sandra W. Clifton And Makedonka Mitreva -- Construction Of Cdna Libraries : Focus On Protists And Fungi / Naiara Rodríguez-ezpeleta [and Others] -- Generation Of Full-length Cdna Libraries : Focus On Plants / Motoaki Seki [and Others] -- Preparation Of Full-length Cdna Libraries : Focus On Metazoans / Masako Harada And Yoshihide Hayashizaki -- Expressed Sequence Tags : Normalization And Subtraction Of Cdna Libraries / Marcelo Bento Soares [and Others] -- Generating Est Libraries : Trans-spliced Cdnas / Cecilia Fernández And Rick M. Maizels -- Large-scale Sequencing And Analytical Processing Of Ests / Makedonka Mitreva And Elaine R. Mardis -- Est Processing : From Trace To Sequence / Ralf Schmid And Mark Blaxter -- Obtaining Accurate Translations From Expressed Sequence Tags / James Wasmuth And Mark Blaxter -- Est Databases And Web Tools For Est Projects /yao-qing Shen [and Others] -- Phylogenomic Analysis Of Est Datasets / José M. Peregrín-alvarez And John Parkinson -- Statistical Analysis Of Expressed Sequence Tags / Edward Susko And Andrew J. Roger -- Application Of Ests In Microarray Analysis / Weizhong Li [and Others] -- Exploiting Ests In Human Health / Sandro José De Souza. Edited By John Parkinson. Includes Bibliographical References And Index. Front Matter....Pages i-x Back Matter....Pages 1-12 ....Pages 13-32
