The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In __Functional Glycomics: Methods and Protocols__, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful __Methods in Molecular Biology__TM series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, __Functional Glycomics: Methods and Protocols__ serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field. Functional glycomics and glycobiology : an overview / Jianjun Li and James C. Richards Historical overview of glycoanalysis / Alicia M. Bielik and Joseph Zaia Quantitative glycomics / Ron Orlando Human gangliosides and bacterial lipo-oligosaccharides in the development of autoimmune neuropathies / Nobuhiro Yuki Biotinylated multivalent glycoconjugates for surface coating / Alexander A. Chinarev, Oxana E. Galanina, and Nicolai V. Bovin Profiling LPS glycoforms of non-typeable Haemophilus influenzae by multiple-stage tandem mass spectrometry / Elke K.H. Schweda and James C. Richards In vitro reconstitution of Escherichia coli O86 O antigen repeating unit / Weiqing Han ... [et al.] Glycoprotein characterization / Susan M. Twine, Luc Tessier, and John F. Kelly N-linked glycoprotein analysis using dual-extraction ultrahigh-performance liquid chromatography and electrospray tandem mass spectrometry / S.O. Siu ... [et al.] Microarray-based study of carbohydrate-protein binding / Zhenxin Wang and Jingqing Gao The application of NMR spectroscopy to functional glycomics / Jean-Robert Brisson ... [et al.] Metabolomics in glycomics / Evelyn C. Soo and Joseph P.M. Hui Characterization of lipid-linked oligosaccharides by mass spectrometry / Christopher W. Reid, Jacek Stupak, and Christine M. Szymanski A glycomics approach to the discovery of potential cancer biomarkers / Hyun Joo An and Carlito B. Lebrilla Extraction of chondroitin/dermatan sulfate glycosaminoglycans from connective tissue for mass spectrometric analysis / Alicia M. Bielik and Joseph Zaia N-linked protein glycosylation in a bacterial system / Harald Nothaft ... [et al.] Characterization of polysaccharides using mass spectrometry for bacterial serotyping / Eleonora Altman and Jianjun Li Is permethylation strategy always applicable to protein N-glycosylation study? : a case study on the O-acetylation of sialic acid in fish serum glycans / Xin Liu and Luis Afonso Bioinformatics in glycomics : glycan characterization with mass spectrometric data using SimGlycan / Arun Apte and Ningombam Sanjib Meitei. The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols , a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology TM series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field. Front Matter....Pages i-xi Back Matter....Pages 1-8 ....Pages 9-30
