Genetic Engineering: Principles and Methods / Volume 28 (Genetic Engineering: Principles and Methods)

**Genetic Engineering: Principles & Methods** **Jane K. Setlow**, PhD __Brookhaven National Laboratory, Upton, NY, USA__ **About the Series:** __Genetic Engineering: Principles and Methods__ presents state-of-the-art discussions in modern genetics and genetic engineering. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among many other topics. **Volume Key Features:** __Genetic Engineering: Principles and Methods__, Volume 28 contains discussions of contemporary and relevant topics in genetics, including: * Regulation of Plant Intracellular Communication Via Plasmodesmata * Root-knot and Cyst Nematode Parasitism Genes: The Molecular Basis of Plant Parasitism * Mutagenesis of Human p53 Tumor Supressor Gene Sequences in Embryonic Fibroblasts of Genetically Engineered Mice * Salicylic Acid, Jasmonic Acid and Ethylene-Mediated Regulation of Plant Defense Signaling * Proximity Ligation: A Specific and Versatile Tool for the Proteomic Era * Protein Overexpression in Mammilian Cell Lines * A High-Throughput Approach to Protein Structure Analysis * New Mass-Spectrometry-Based Strategies for Lipids * Paired-End Genomic Signature Tags: A Method for the Functional Analysis of Genomes and Epigenomes * All chapter titles are original content by the authors This principles and methods approach to genetics and genetic engineering is essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field. About the Editor: Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York. Genetic Engineering: Principles and Methods presents state-of-the-art discussions in modern genetics and genetic engineering. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among many other topics. Volume Key Features: Genetic Engineering: Principles and Methods, Volume 27 contains discussions of contemporary and relevant topics in genetics, including: Identification and Analysis of Micrornas Dormancy and the Cell Cycle Long distance peptide and metal transport in plants Signaling in plant response to temperature and water stresses Nutrient transport and metabolism in plants Salt Stress Signaling and Mechanisms of Plant Salt Tolerance Gene cloning and expression Assisted folding and assembly of proteins This principles and methods approach to genetics and genetic engineering is essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field. Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York

this Volume, As With The Previous Books In The Series, Presents State-of-the-art Discussions In Genetics And Genetic Engineering By Focusing On Plant Science And Technology, Agriculture, Cell Biology, And Medical Research.

roger W. Melvold

this Is The 17th Edition Of An Annual Volume Of Contributed Papers In Various Areas Of Genetic Engineering. It Is Intended To Present Up-to-date Information In The Field. This Volume Will Be Of Interest To Investigators Interested In Genetic Manipulation And Engineering And Would Also Be Of Value To Library Collections. The Individual Contributions Are Well Illustrated And Include Extensive Pertinent References. Both Eukaryotic And Prokaryotic Organisms Are Included. The 13 Entries In This Volume Include Coverage Of Dna Excision And Repair, Bacterial Virulence Genes, Metal Binding Proteins, Rna Tertiary Structure, Expression Systems, Toxins, And Genetic Exchanges Between Plant Cells And Bacteria. Several Of The Entries Deal Heavily With Technical Developments. This Annual Series Of Reviews Is Strong On Technique.

Front Matter....Pages i-xxii Regulation of Plant Intercellular Communication Via Plasmodesmata....Pages 1-15 Root-Knot and Cyst Nematode Parasitism Genes: The Molecular Basis of Plant Parasitism....Pages 17-43 Mutagenesis of Human p53 Tumor Suppressor Gene Sequences in Embryonic Fibroblasts of Genetically-Enginered Mice....Pages 45-54 Salicylic Acid-, Jasmonic Acid- and Ethylenemediated Regulation of Plant Defense Signaling....Pages 55-83 Proximity Ligation: A Specific and Versatile Tool for the Proteomic Era....Pages 85-93 Protein Overexpression in Mammalian Cell Lines....Pages 95-104 A High-Throughput Approach To Protein Structure Analysis....Pages 105-128 New Mass-Spectrometry-Based Strategies For Lipids....Pages 129-157 Paired-End Genomic Signature Tags: A Method for the Functional Analysis of Genomes and Epigenomes....Pages 159-173 Back Matter....Pages 175-190 Genetic Engineering: Principles and Methods, published by Springer since 1979, presents state-of-the-art discussions in modern genetics and genetic engineering. This focus affirms a commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among other topics. This book, published by Springer since 1979, presents state-of-the-art discussions in modern genetics and genetic engineering. This focus affirms a commitment to publish important reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among other topics.