While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens. This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutionsContent: Chapter 1 Vaccination: Past, Present and Future (pages 1–22): Maria Lattanzi and Rino RappuoliChapter 2 Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies? (pages 23–41): Guido GrandiChapter 3 Genome Sequencing and Analysis (pages 43–73): Herve Tettelin and Tamara FeldblyumChapter 4 Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets (pages 75–101): She?Pin Hung, Suman Sundaresh, Pierre F. Baldi and G. Wesley HatfieldChapter 5 The Proteome, Anno Domini Two Zero Zero Three (pages 103–134): Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joel S. RossierChapter 6 Mass Spectrometry in Proteomics (pages 135–169): Pierre?Alain BinzChapter 7 High?Throughput Cloning, Expression and Purification Technologies (pages 171–182): Andreas Kreusch and Scott A. LesleyChapter 8 Meningococcus B: From Genome to Vaccine (pages 183–204): Davide Serruto, Rino Rappuoli and Mariagrazia PizzaChapter 9 Vaccines against Pathogenic Streptococci (pages 205–222): John L. Telford, Immaculada Margarit Y Ros, Domenico Maione, Vega Masignani, Herve Tettelin, Giuliano Bensi and Guido GrandiChapter 10 Identification of the ‘Antigenome’ — A Novel Tool for Design and Development of Subunit Vaccines against Bacterial Pathogens (pages 223–243): Eszter Nagy, Tamas Henics, Alexander von Gabain and Andreas MeinkeChapter 11 Searching the Chlamydia Genomes for New Vaccine Candidates (pages 245–266): Giulio Ratti, Oretta Finco and Guido GrandiChapter 12 Proteomics and Anti?Chlamydia Vaccine Discovery (pages 267–283): Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C ShawChapter 13 Proteome Analysis of Outer Membrane and Extracellular Proteins from Pseudomonas aeruginosa for Vaccine Discovery (pages 285–304): Stuart J. Cordwell and Amanda S. Nouwens
While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly. Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens. This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions
The complete genome sequences of many bacteria are now available, and comparisons of pathogenic strains with non-pathogenic relatives are becoming more common. This will enable researchers to target vaccine development towards specific gene products that traditional approaches of vaccine research failed to discover. Today, there are several examples of vaccine candidate identification using genomics and proteomics, making "Genomics, Proteomics, and Vaccines's state-of-the-art picture of the field and summary of the possible strategies extremely useful
