HIV Protocols (Methods in Molecular Biology)

Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular BiologyTM series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1. Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1. Front Matter....Pages i-xiv Front Matter....Pages 1-1 Front Matter....Pages 3-14 Front Matter....Pages 15-25 Front Matter....Pages 27-35 Front Matter....Pages 36-37 Front Matter....Pages 41-53 Back Matter....Pages 55-72 ....Pages 73-85