In-Vitro Fertilization

This extensively updated new edition provides an indispensable account of modern in-vitro fertilization practice, building upon the popularity of previous editions. The authors initially give a comprehensive review of the biology of human gametes and embryos, before outlining basic to advanced IVF techniques. New developments in practical techniques and understanding are discussed, including in-vitro maturation, vitrification, preservation of fertility for cancer patients, stem cell technology, preimplantation genetic testing, and the role of epigenetics and imprinting. The revised introduction also incorporates a 'refresher' study review of fundamental principles of cell and molecular biology, now updated with current knowledge of meiosis in human oocytes, embryo metabolism and basic principles of genome editing. With high-quality illustrations and extensive, up-to-date reading lists, it is a must-have textbook for trainee and practising embryologists, as well as clinicians who are interested in the scientific principles that underpin successful IVF. Review of cell and molecular biology -- Endocrine control of reproduction: controlled ovarian hyperstimulation for ART -- Gametes and gametogenesis -- Sperm-oocyte interaction -- First stages of development -- Implantation and early stages of fetal development -- Stem cell biology -- The clinical in-vitro fertilization laboratory -- Quality management in the IVF laboratory -- Sperm and ART -- Oocyte retrieval and embryo culture -- Cryopreservation of gametes and embryos -- Micromanipulation techniques -- Preimplantation genetic diagnosis -- Epigenetics and assisted reproduction Fully updated new edition providing an indispensable account of modern IVF practice. Now includes recent advances in practical techniques, from in-vitro maturation and vitrification to stem cell technology. Illustrations and up-to-date reading lists make this a must-have textbook for trainee and practising embryologists working in IVF Units.