Introduction to Proteomics

Introduction to Proteomics presents an overview of proteomics, including a discussion of the field's fundamental ideas, how to use various technologies and instruments, and examples of applications in human health and illness. This textbook provides new students and researchers with an easy-to-grasp, comprehensive review and knowledge of the concepts, guidelines, and especially the difficult instrumentation procedures in proteomics. Cover 1 Title Page 5 Copyright 6 ABOUT THE AUTHOR 7 TABLE OF CONTENTS 9 List of Figures 13 List of Tables 17 Preface 19 Chapter 1 Fundamentals of Proteomics 21 1.1 Introduction 22 1.2 Proteins that Perform Particular Functions 27 1.3 Pregenomic Proteomics 28 1.4 Genetics of Proteins 30 1.5 One Gene-Many Protein: Challenge to Proteomics 37 1.6 RNA Silencing and Proteomics 43 1.7 Molecular Biology of Genes and Proteins 45 1.8 Protein Chemistry Before Proteomics 50 1.9 Unstructured Protein 62 1.10 Protein Misfolding and Human Disease 64 References 67 Chapter 2 Proteomics—Relation to Genomics 79 2.1 Introduction 80 2.2 Genomics 80 References 100 Chapter 3 Methodology for Separation and Identification of Proteins and Their Interactions 107 3.1 Introduction 108 3.2 Separation of Protein Via the Multidimensional Approach 108 3.3 Determination of the Primary Structure of Proteins 114 3.4 Determination of the 3D Structure of a Protein 123 3.5 Determination of the Number of Proteins 128 3.6 Structural and Functional Proteomics 133 References 139 Chapter 4 Principles of Liquid Chromatography In Proteomics 149 4.1 Introduction 150 4.2 General Chromatographic Principles for Peptide and Protein Segregation 150 4.3 Affinity Chromatography 153 4.4 Ion Exchange Chromatography 154 4.5 Reversed-Phase Chromatography 155 4.6 Size Exclusion Chromatography 158 4.7 Multidimensional Liquid Chromatography 159 References 163 Chapter 5 Proteomics of Protein Modifications 167 5.1 Introduction 168 5.2 Phosphorylation and Phosphoproteomics 169 5.3 Glycosylation and Glycosylation 173 5.4 Ubiquitination and Ubiquitinomics 178 5.5 Miscellaneous Modifications of Proteins 181 References 184 Chapter 6 Proteomics of Protein-Protein Interactomes 189 6.1 Introduction 190 6.2 Protein-Protein Interactions In Vivo 191 6.3 Analysis of Protein Interaction In Vitro 195 6.4 Analysis of Protein Interactions In Silico 200 6.5 Interactomes 202 6.6 Evolution and Conservation of Interactions 210 6.7 Interaction of Proteins with Small Molecules 211 References 212 Chapter 7 Applications of Proteomics 219 7.1 Introduction 220 7.2 Diseasome 221 7.3 Medical Proteomics 222 7.4 Clinical Proteomics 227 7.5 Metaproteomics and Human Health 231 References 234 Chapter 8 Developments in Proteomics 239 8.1 Introduction 240 8.2 Technical Scope of Proteomics 240 8.3 Scientific Scope of Proteomics 242 8.4 Medical Scope of Proteomics 244 8.5 Proteomics, Energy Production, and Bioremediation 250 8.6 Proteomics and Biodefense 250 References 252 Index 257 Back Cover 261