Manipulation of Mammalian Development

Developmental biology has been transformed recently by discoveries in the fields of molecular biology, cell biology, and immunology. New ways of manip­ ulating mammalian development are uncovering control mechanisms and ena­ bling us to apply them in solving practical problems in animal production and human health. This book outlines some of these new manipulations and how they have contributed to the present state of developmental biology. Chapter 1 describes gene transfer by micro injection of cloned recombinant DNA into zygotes. Although the factors that affect transformation frequencies and integration sites are still unknown, such techniques offer a number of exciting prospects. Research models for human disease coula be artificially created and desirable characteristics in agricultural animals could be - hanced. . The theme of cell-to-cell transfer is continued in Chapters 2 and 3. Chapter 2 describes pronuclear transplantation by Sendai virus-induced fusion of the karyoplast with the enucleated embryo. Using this procedure, it has been dem­ onstrated that both male and female genomes are essential for normal develop­ ment, although the reason for this is not yet understood. Chapter 3 describes studies on the fusion of whole oocytes. . Front Matter....Pages i-xiv Gene Transfer into Mouse Embryos....Pages 1-36 Nuclear and Cytoplasmic Transfer in Mammalian Embryos....Pages 37-55 Oocyte Fusion....Pages 57-80 Animal in Vitro Fertilization and Embryo Development....Pages 81-148 Recent Progress in Human in Vitro Fertilization and Embryo Transfer....Pages 149-194 Embryo Sexing of Farm Animals....Pages 195-216 Cryopreservation of Mammalian Eggs and Embryos....Pages 217-247 Embryo Transfer in Animals....Pages 249-278 Manipulation of Early Mammalian Development: What Does It Tell Us about Cell Lineages?....Pages 279-296 Tissue Interactions in Development of Teeth and Related Ectodermal Derivatives....Pages 297-313 Tissue Combination and Organ Culture Studies in the Development of the Embryonic Mammary Gland....Pages 315-333 Immunogenetic Approaches to the Analysis of Mammalian Development....Pages 335-352 The Immunological Approach to the Study of Preimplantation Mammalian Development....Pages 353-381 Back Matter....Pages 383-388