Manual on Membrane Lipids (Springer Lab Manuals)

Although previously thought to be merely passive structural components, membrane lipids have recently been found to be actively involved in cellular transport and signal transduction processes. Clear protocols for the study of membrane lipid properties, cellular transport or signal transduction are presented in this manual. Following a short introduction to membrane lipids, techniques for the isolation and extraction of membrane fractions, the analysis of the lipid composition, lipid turnover, and the involvement in signal transduction as well as the preparation of liposomes are described. Front Matter....Pages I-XIX Structure and Distribution of Membrane Lipids....Pages 1-15 Isolation of Pure Membrane Fractions for Lipid Analysis....Pages 16-36 Extraction of Membrane Lipids....Pages 37-51 Chromatographic Analysis of Lipids....Pages 52-79 Phase Transition of Membrane Lipids....Pages 80-111 Lipid Asymmetry of Membranes....Pages 112-143 Turnover of Lipids....Pages 144-166 Lipids and Signal Transduction....Pages 167-201 Liposomes: Preparation and Membrane Protein Reconstitution....Pages 202-247 Back Matter....Pages 248-255 Although previously thought to be merely passive structural components, membrane lipids have recently been found to be actively involved in cellular transport and signal transduction processes. This manual describes the techniques for analyzing membrane lipids.