There are currently a growing number of laboratories actively studying the mechanism by which various biological membranes are assembled. This area of research is still relatively new to biochemists and molecular biologists, but in view of the rapid progress being made, a review of the field at this time is justified. The present volume focuses on the biogenesis of three related membranes. Mitochondria and chloroplasts are semiautonomous organelles whose biogenesis is carried out partly in the external cytoplasm and partly by the organelles themselves. Both membranes are principally concerned with the energy metabolism of the cell, and this commonality of function is reflected in a considerable degree of similarity in their ul trastructure and enzymatic composition. Although the bacterial cell membrane is a much more diversified structure, it also fulfills the basic energy requirements of the cell, and depending on the organism, this can take the form of photosynthesis or oxidative phosphorylation. The addi tional consideration that prokaryotic organisms may, in fact, be the evolu tionary ancestors of mitochondria and chloroplasts, makes it all the more compelling that those interested in biogenesis be aware of new develop ments in each of these three areas. In organizing this book, I felt that the contributors should summarize and bring up to date their own research and review the literature only in sofar as would be necessary to provide the proper perspective for their work. Front Matter....Pages i-xvii Current Problems in Membrane Biogenesis....Pages 1-14 Biogenetic Autonomy of Mitochondria and Its Limits....Pages 15-61 Stable Pleiotropic Respiratory-Deficient Mutants of a “Petite-Negative” Yeast....Pages 63-97 Biogenesis of the Yeast Mitochondrial Membranes....Pages 99-124 Biogenesis of Cytochrome Oxidase and Cytochrome b in Neurospora crassa ....Pages 125-153 Nuclear-Cytoplasmic Interactions in the Biogenesis of Mitochondria in Yeast....Pages 155-199 Eukaryotic Intracellular Relationships....Pages 201-245 The Synthesis of Chloroplast Membranes in Pisum sativum ....Pages 247-278 Biogenesis of Chloroplast Membranes....Pages 279-350 Biosynthesis and Assembly of the Outer Membrane Proteins of Escherichia coli ....Pages 351-391 Role of Adhesion Zones in Bacterial Cell-Surface Function and Biogenesis....Pages 393-427 Studies on Bacterial Membrane Biogenesis Using Glycerol Auxotrophs....Pages 429-454 Back Matter....Pages 455-460 1 Current Problems in Membrane Biogenesis.- 1. Organization of Protein and Lipid Components of Membranes.- 1.1. Lipid Components.- 1.2. Protein Constituents.- 2. General Aspects of Membrane Growth.- 2.1. Integration of Hydrophobic Proteins.- 2.2. Assembly of Membrane Enzymes.- 2.3. Integration Sites of Membranes.- 2.4. Integration of Lipids.- 3. Some Problems in Organelle and Bacterial Envelope Biogenesis.- 3.1. Mitochondria and Chloroplasts.- 3.2. Bacterial Envelope.- 4. Concluding Remarks.- 5. References.- 2 Biogenetic Autonomy of Mitochondria and Its Limits.- 1. Introduction.- 2. Limits on
