Nuclear Structure and Function

This collection of 101 short communications, submitted by some of the participants at the 11th Nuclear Workshop held in Suzdal, USSR, 18-23 September, 1989, provides a representative survey of the material presented at the Workshop. Articles have been submitted by both those who delivered lectures and those who had poster presentations. The order of presentation at the Nuclear Workshop is roughly maintained within this proceedings book, but the session titles within the scientific program have not been utilized as discrete subdivisions within the book, because of the considerable overlap of subject matter. The overall sequence is as follows: Genome structure, Gene Structure and Expression, Nucleolar Genes, Structure and Proteins, Chromatin and Nuclear Granules, Nuclear Matrix and Nuclear Proteins, Replication and Transcription and finally Nuclear Envelope and Nuclear Cytoplasmic Transport. Several articles on Nuclear Lipids are also included, stemming from an evening round-table discussion on lipids. The third Wilhelm Bernhard Lecture was delivered in Suzdal by Professor Harris Busch, who can be seen in the photograph above (on the left) in the presence of Professor Ilya B. Zbarsky, President of the organizing committee for the 11th Nuclear Workshop. (Previous Wilhelm Bernhard lecturers have been Ronald H. Reeder, in Krakow, Poland, 1985 and Oscar L. Miller, Jr. , in Stevensbeek, The Netherlands, in 1987). Front Matter....Pages i-xv The Putative Transposable Element T14 is Located on a Y Chromosomal Lampbrush Loop and Other Genomic Sites and is Transcribed in the Germ Line of Drosophila Hydei ....Pages 1-10 DNA Sequence-Specific Location of Covalent DNA-Polypeptide Complexes in Eukaryotic Genomes....Pages 11-14 Mink Sau Repeat: Structural Analysis and Genome Distribution....Pages 15-18 Structure of the Plant Mitochondrial Genome and Light-Regulated Transcription of the Mitochondrial Genes....Pages 19-22 Role of Damage of Skeletal Structures in the Induction of Chromosome Aberrations....Pages 23-26 Possible Functional Structures in the Chromomere....Pages 27-30 Rat DNA Fingerprinting for Rattus Norvegicus : A New Approach in Genetic Analysis....Pages 31-35 The Extracellular Lymphocyte and Blood Plasma DNAs Contain the Discrete Size Molecules Homologous to the C k Fragment of the Ig Gene....Pages 37-40 Non-Random Distribution of Alu-Family DNA Repeats in Human Chromosomes....Pages 41-45 Isolation and Properties of a Novel Specific Fraction of Chromosomal DNA from Human, Drosophila and Plant Cells....Pages 47-51 Structural Elements of Balbiani Ring BRa Of Chironomus Thummi ....Pages 53-56 Role of Specific Protein S-S Bonds in the Quasisubunit Structure of Chromosomal DNA....Pages 57-60 An Aspect of Genome Variability: Retrotransposons (MDG1 Copies) in the Euchromatin and Heterochromatin of Drosophila Melanogaster ....Pages 61-65 IS Elements in Agrobacterium Temefaciens Strains and Their Putative Implications in Horizontal Gene Transfer to Plants....Pages 67-72 Functional Analysis of the Nucleolin Gene Promoter from the Mouse....Pages 73-78 Expression of Ribosomal Protein Genes in Xenopus Development....Pages 79-82 Ribosomal Protein Genes in Xenopus Laevis: Organization, Structure and Identification of the cis-Element Responsible for Their Translational Control....Pages 83-87 Expression of Nucleoprotein mRNAs During Rat Spermiogenesis....Pages 89-93 Splicing Control and Nucleus/Cytoplasm Compartmentalization of Ribosomal Protein L1 RNA in X. Laevis Oocytes....Pages 95-98 Genetic Analysis of Spermatogenesis in Drosophila Hydei : Male Sterile Mutations Affecting Nuclear Development During the Primary Spermatocyte Stage....Pages 99-103 The Study of the Influence of cis-Regulatory Sequences and trans-Regulatory Factors of Eucaryotes on CAT Gene Expression in X. Laevis Oocytes....Pages 105-108 The Detection of Noncanonical Conformation Regions in DNA From Eukaryotic Cells....Pages 109-113 Characterization of Cytoplasmic Low-Molecular-Mass RNA Complementary Associated with Poly(A) + RNA from Rat Liver....Pages 115-118 In Vitro Amplification of the Human Insulin Gene....Pages 119-123 Chromosomes E and S in the Oogenesis of the Gall Midge....Pages 125-128 Nuclear Reprogramming in Bovine Embryos after Nuclear Transplantation....Pages 129-132 Cell Differentiation According to Maturation of Nucleolar Apparatus and Topography of Ribosomal Genes....Pages 133-137 Gene Families Coding for the Eye Lens Proteins of Cephalopods....Pages 139-141 Loach (Misgurnus Fossilis) Oocyte 5S rRNA Genes: Heterogeneity of Primary Structure and Location of a Transcription Stimulatory Signal in Their Upstream Spacer....Pages 143-147 Nucleolar DNA Distribution and Its Consequences for the Interpretation of Nucleolar Components....Pages 149-152 Effects of Topoisomerase I Inhibition on Nucleolar Structure and Function....Pages 153-156 Localization of DNA and Characterization of Argyrophilic Structures within Nucleolar Components during Interphase and Mitosis in Leukemia Cell Lines....Pages 157-160 Relationship between the Ag-Nor Proteins and the Functional Changes in Nucleoli of the Rat Hepatocytes Stimulated by Cortisol and by Partial Hepatectomy....Pages 161-164 Origin and Ultrastructural Cytochemistry of the Extranucleolar Bodies at the Preovulatory Follicle Stage and during Rat Oocyte Maturation....Pages 165-168 Spatial Distribution of DNA and Histones within Ehrlich Tumor Cell Nuclei by Immunoelectron Microscopy....Pages 169-171 Electron Microscope Localization of Ribosomal RNA and DNA after in Situ Hybridization....Pages 173-176 DNA Methylation and Experimentally Induced Nucleolar Dominance....Pages 177-177 Peculiarities of Silver-Staining of Nucleolar Organizer Regions in Chromosomes of Human Permanent Cell Lines at the Light Microscopical and Ultrastructural level....Pages 179-181 Morphofunctional Characteristics of Silver-Stained Nucleoli in Human Normal and Pathological Cells....Pages 183-185 Nucleologenesis in Cow Embryo: Relation between Onset of Transcription and Penetration of DNA into Nucleolar Precursor Body....Pages 187-191 Structural Transitions in Mammalian Cell Nucleoli Concomitant to Ribosomal Gene Activation and Inactivation....Pages 193-196 Nucleolar Changes in Hypotonic Treatment and the Possibility of Isolation of Nucleolar Fibrillar Complexes....Pages 197-202 Transcriptionally Active Amphibian Oocyte Nucleolar Chromatin is Organized in Higher Order Structure....Pages 203-206 Nucleolar Organizing Region (NOR) and Argentophilic Nuclear Proteins in Fertilization and Induced Parthenogenesis....Pages 207-210 The Competitive Regulation of Nucleolar Activity in an Interphase Nucleus Revealed by UV-Microirradiation of the Nucleoli....Pages 211-214 Ag-Positive Nucleolar Organizer Regions in Interphase Blast Cells in Acute Leukemias....Pages 215-218 Polymorphism and the Regularities in the Inheritance of Human Nucleolus Organizer Chromosomes....Pages 219-222 Satellite Nucleoli in Blastic Cells of Human Acute Leukemias....Pages 223-225 Studies on the Distribution and Cytochemistry of Nucleolar Silver Stained Proteins in Ring Shaped Nucleoli....Pages 227-230 Implications for the Function-Structure Relationship in the Nucleolus After Immunolocalization of DNA in Onion Cells....Pages 231-235 Biotechnology and Human Tumor Nucleolar Antigens....Pages 237-242 Origin of Isoforms and Interaction with Nucleic Acids....Pages 243-246 Nucleolin Maturation and rRNA Synthesis during Xenopus Laevis Development....Pages 247-252 Localization of Non-Histone Nuclear Proteins by Immunocytochemistry in Somatic Embryos and Pollen Grains....Pages 253-258 The ‘Argentaffin’ Reaction of the Nucleolus. Silver Reducing Sites after Mercuric Acetate and Copper Tetrammine Treatments....Pages 259-264 Immunochemical Identification of a Novel Nuclear RNP Complex of 70-11OS from Rat Liver....Pages 265-272 Visualization of Chromatin Arrangement in Giant Nuclei of Mouse Trophoblast by Videomicroscopy and Laser Scanning Microscopy....Pages 273-277 Effects of a DNA Intercalator, Ethidium Bromide on Chromatin Structure of Chicken Erythrocyte....Pages 279-284 The Fine Structure of Native DNA Studied with the Scanning Tunneling Microscope....Pages 285-289 Cytochemistry and Immunology of some Nuclear Proteins in a Primative Dinoflagellate....Pages 291-296 Higher Order Chromatin Structure in Transcriptionally Active and Inactive Cells....Pages 297-300 Chromomere — The Structural Unit of the Chromatin in the Interphase Nucleus....Pages 301-304 An Immunoelectronmicroscopic Analysis of the Organization Levels of the Metaphase Chromosomes....Pages 305-307 The Effect of Sulfhydryl-Oxidizing and Sulfhydryl-Blocking Reagents on Chromatin Preparations....Pages 309-311 Fractionation and Analysis of DNA Molecules Differing in Their Attachment to the Nuclear Interior....Pages 313-316 Plant Nuclear Matrix Effects of Different Extraction Procedures on its Structural Organization and Chemical Composition....Pages 317-322 Synthesis and Phosphorylation of the 125 K Nuclear Matrix Protein Mitotin during the Cell Cycle....Pages 323-328 Identification of Myosin-Like Proteins in Cell Nuclei, their Interaction with Chromatin Components....Pages 329-332 DNA Sequences Nonrandomly Distributed in Respect to Rat Liver Nuclear Matrix....Pages 333-336 DNA-Folding by a Stably DNA-Linked Protein in Eukaryotic Chromatin....Pages 337-339 Structural Changes of Nucleosomes at the Sites of Non-Ribosomal RNA Transcription....Pages 341-344 A Fragment of Chicken Nuclear Matrix-Associated DNA Can Maintain Autonomous Replication of Plasmids in Mammalian Cells....Pages 345-350 Histone Hi-Specific and DNA-Activated Proteinase Is Associated with the Nuclear Matrix from RAT Liver....Pages 351-354 High Molecular Weight Proteins of Tumor Nuclear Matrix....Pages 355-359 MAP-2-Like DNA Binding Protein: Monoclonal Antibody Recognizes Ubiquitous Protein of Internal Filaments of Nuclear Matrix....Pages 361-364 Disconnection of DNA Domains in Quiescent and Differentiating Cells....Pages 365-369 Heat Shock Proteins Associated with Nuclear Structures....Pages 371-375 Characterization of the DNA Pattern in the Vicinity of a Replication Origin Located Upstream from the Domain of Chicken α-Globin Genes....Pages 377-382 Parameters of Xenopus rDNA Transcription in Microinjected Oocytes....Pages 383-388 Transcriptional Activity of the 5′ Region of the Xenopus Laevis R-Protein Gene L1....Pages 389-392 Correlation between Changes in Sphingomyelinase Activity and Level of Nucleic Acid Synthesis in Cell Nuclei of Regenerating Rat Liver....Pages 393-397 Role of Lipids in Functional Activity of Cell Nucleus....Pages 399-403 Roles of DNA Topoisomerases I and II in DNA Replication, Mitotic Chromosome Formation, and Recombination in Mammalian Cells....Pages 405-411 Effects of DRB on the Ultrastructural Organization and RNA Metabolism of Plant Nucleus....Pages 413-418 Replication Time of DNA Sequences Related to G 1 Progression in Meristematic Cells....Pages 419-419 Nuclear Liver Protein Interaction with the Rat Tryptophan Oxygenase (TO) Gene Fragments Including the DNase I Hypersensitive Sites ‘Poised’ for Transcriptional Induction....Pages 421-424 The snRNA and Regulation of the Early Development in Teleosts....Pages 425-427 Differences in Transcription Activity and Replication Time of rRNA Genes in a Pair of Heteromorphic Nucleolar Organizing Chromosomes in Transformed Kidney Cells of the African Green Monkey (RAMT Strain)....Pages 429-432 Reactivation of Latent Nuclear Polyhedrosis Virus in Silkworm....Pages 433-436 New Structural Elements of Poly(A)—Containing RNAs in Mouse Cells....Pages 437-442 Gibberellin-Binding Proteins of Higher Plant Cell Nuclei....Pages 443-446 Developmental Expression of Proto-Oncogene c- erb -A Related Genes Encoding Nuclear Receptors in Xenopus ....Pages 447-451 The Secondary Structure Model of Mouse U1 snRNA as Determined from the Results of Pb-Induced Hydrolysis....Pages 453-457 Age-Specific Differences in the Metabolism of Nuclear Phospholipid Fatty Acid Components....Pages 459-461 Changes in Lipid Metabolism in Rat Liver Cell Nuclei During Superexpression of Nuclear Oncogenes....Pages 463-467 Role of Phospholipids on Nuclear Structure and Function....Pages 469-471 The Chromatoid Body in Spermatogenesis: Nucleo-Cytoplasmic Transport of Haploid Gene Products and Its Cytoskeletal Regulation....Pages 473-476 Selective Staining of DNA at the Ultrastructural Level After Alkaline Hydrolysis....Pages 477-481 Ultrastructural Changes in Nuclei Within HIV-1-Induced Cultured Cell Syncytia....Pages 483-487 An Electron Microscopic Study of the Structural Mechanisms of Hybrid Nucleus Formation During Spontaneous Splenocyte-Fibroblast Fusion....Pages 489-492 Nuclear Compartmentalization in Pollen Mother Cells During Meiotic Prophase....Pages 493-497 Back Matter....Pages 499-504