Nuclear Structures : Isolation and Characterization

Nuclear Structures: Isolation and Characterization focuses on techniques for the isolation of molecular and macromolecular components of cell nuclei. The book first discusses nuclear matrices, including isolation of nuclear matrices and notes on the method. The text examines nucleoids. Nucleoid preparation and morphology, characterization of nucleoids, experimental uses of nucleoids, and criticism of nucleoid preparation are underscored. The book also discusses nuclear envelopes, polynucleosomes and monomer nucleosomes, and metaphase chromosomes. The text also examines DNA tight-binding proteins, including the definition and preparation of DNA tight-binding proteins; immunological characterization of tight-binding proteins; and DNA binding characteristics. The book also examines nucleoli and preribosomal ribonucleoprotein particles. Methods for the isolation of nucleoli; assessment of nucleolar preparations; and isolation of preribosomal ribonucleoprotein particles are discussed. The text also covers ribonucleoprotein particles containing heterogeneous nuclear RNA and nuclear RNP particles containing small RNAs. The selection is a good reference for readers wanting to study the isolation of molecular and macromolecular components of cell nuclei. Content: Inside Front Cover , Page ii Front Matter , Page iii Copyright , Page iv Preface , Pages v-vi , A.J. MacGillivray, G.D. Birnie Contributors , Pages vii-viii Chapter 1 - Nuclear matrices , Pages 1-13 , Shona A. Comerford, Paul S. Agutter, Alexander G. McLennan Chapter 2 - Nucleoids , Pages 14-33 , Dean A. Jackson Chapter 3 - Nuclear envelopes , Pages 34-46 , Paul S. Agutter Chapter 4 - Polynucleosomes and monomer nucleosomes , Pages 47-73 , J. Allan, R.H. Nicolas, G.H. Goodwin Chapter 5 - Metaphase chromosomes , Pages 74-85 , Bryan D. Young Chapter 6 - DNA tight-binding proteins , Pages 86-99 , Ailsa M. Campbell, Caroline V.P. Addey Chapter 7 - Nucleoli and preribosomal ribonucleoprotein particles , Pages 100-117 , Trevor J.C. Beebee Chapter 8 - Ribonucleoprotein particles containing heterogeneous nuclear RNA , Pages 118-129 , J.T. Knowler, C.W. McGregor, Z. Islam Chapter 9 - Nuclear RNP particles containing small RNAs , Pages 130-162 , Alexander J. MacGillivray Chapter 10 - Hormone receptors , Pages 163-181 , Robin Leake Chapter 11 - DNA and RNA , Pages 182-201 , G.D. Birnie, S.V. Graham Index , Pages 203-209 Edited By A.j. Macgillivray And G.d. Birnie. Includes Bibliographies And Index.