Oncolytic Viruses

This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Oncolytic Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field Front Matter ....Pages i-xi Introduction to Oncolytic Virotherapy (Christine E. Engeland, John C. Bell)....Pages 1-6 Methods for Modification of Therapeutic Viruses (Claudia A. P. Hill, Luca Bau, Robert Carlisle)....Pages 7-29 Tumor Targeting of Oncolytic Adenoviruses Using Bispecific Adapter Proteins (Julia Niemann, Florian Kühnel)....Pages 31-49 Development of Entry-Targeted Oncolytic Measles Viruses (Michael D. Mühlebach, Roberto Cattaneo)....Pages 51-75 Insert Stability and In Vivo Testing of MicroRNA-Detargeted Oncolytic Picornaviruses (Autumn J. Schulze)....Pages 77-94 Ex Vivo Virotherapy with Myxoma Virus to Treat Cancer (Nancy Y. Villa, Lina S. Franco, Grant McFadden)....Pages 95-110 Immunomodulation in Oncolytic Measles Virotherapy (Laura Dietz, Christine E. Engeland)....Pages 111-126 A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death (Tiphaine Delaunay, Carole Achard, Marc Grégoire, Frédéric Tangy, Nicolas Boisgerault, Jean-François Fonteneau)....Pages 127-132 Design and Production of Newcastle Disease Virus for Intratumoral Immunomodulation (Gayathri Vijayakumar, Dmitriy Zamarin)....Pages 133-154 Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue (Krishna Das, Carles Urbiola, Bart Spiesschaert, Philipp Mueller, Guido Wollmann)....Pages 155-177 Immunohistochemistry for Tumor-Infiltrating Immune Cells After Oncolytic Virotherapy (Dipongkor Saha, Samuel D. Rabkin)....Pages 179-190 Detection of Tumor Antigen-Specific T-Cell Responses After Oncolytic Vaccination (Jonathan G. Pol, Byram W. Bridle, Brian D. Lichty)....Pages 191-211 Evaluation of Oncolytic Virus-Induced Therapeutic Tumor Vaccination Effects in Murine Tumor Models (Rūta Veinalde)....Pages 213-227 Delivery of Oncolytic Reovirus by Cell Carriers (Elizabeth J. Ilett)....Pages 229-236 In Vivo Bioimaging for Monitoring Intratumoral Virus Activity (Liesa-Marie Schreiber, Carles Urbiola, Patrik Erlmann, Guido Wollmann)....Pages 237-248 Oncolytic Immunotherapy for Bladder Cancer Using Coxsackie A21 Virus: Using a Bladder Tumor Precision-Cut Slice Model System to Assess Viral Efficacy (Kate Relph, Nicola Annels, Chris Smith, Marcos Kostalas, Hardev Pandha)....Pages 249-259 Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity (Eleanor M. Scott, Sally Frost, Hena Khalique, Joshua D. Freedman, Len W. Seymour, Janet Lei-Rossmann)....Pages 261-270 Generating Primary Models of Human Cancer to Aid in the Development of Clinically Relevant Oncolytic Viruses (Brian A. Keller, Marie-Ève Wedge, Abera Surendran, Carolina S. Ilkow)....Pages 271-284 Considerations for Clinical Translation of MG1 Maraba Virus (Caroline J. Breitbach)....Pages 285-293 Fluorescence In Situ Hybridization (FISH) Detection of Viral Nucleic Acids in Oncolytic H-1 Parvovirus-Treated Human Brain Tumors (Irina Kiprianova, Alexandra Just, Barbara Leuchs, Jean Rommelaere, Assia L. Angelova)....Pages 295-306 Mathematical Modeling of Oncolytic Virotherapy (Johannes P. W. Heidbuechel, Daniel Abate-Daga, Christine E. Engeland, Heiko Enderling)....Pages 307-320 Back Matter ....Pages 321-329