The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume XXVII, part D Pages ix-x Preface Page xi C.H.W Hirs, Serge N Timasheff Volumes in series Pages xiii-xv [1] Ultracentrifugal studies with absorption optics and a split-beam photoelectric scanner Original Research Article Pages 3-59 H.K Schachman, Stuart J Edelstein [2] Determination of molecular weights in the ultracentrifuge using time-lapse photography Original Research Article Pages 59-67 J.L Bethune [3] Active enzyme centrifugation Original Research Article Pages 67-82 David L Kemper, Johannes Everse [4] Measurement of partial specific volume by sedimentation equilibrium in H 2 OD 2 O solutions Original Research Article Pages 82-98 Stuart J. Edelstein, H.K. Schachman [5] The determination of the partial specific volume of proteins by the mechanical oscillator technique Original Research Article Pages 98-110 O Kratky, H Leopold, H Stabinger [6] Density gradient sedimentation equilibrium Original Research Article Pages 111-127 John E Hearst, Carl W Schmid [7] Proteins in density gradients at sedimentation equilibrium Original Research Article Pages 128-140 James B Ifft [9] Zonal centrifugation Pages 140-150 David Freifelder [9] Small-angle X-ray scattering Original Research Article Pages 151-209 Helmut Pessen, Thomas F. Kumosinski, Serge N. Timasheff [10] Light scattering and differential refractometry Original Research Article Pages 209-256 Eugene P Pittz, James C Lee, Barkey Bablouzian, Robert Townend, Serge N Timasheff [10a] Osmotic pressure Original Research Article Pages 256-269 Guido Guidotti [11] Sedimentation velocity measurement of protein association Original Research Article Pages 273-296 Lilo M Gilbert, G.A Gilbert [12] Measurements of protein interactions mediated by small molecules using sedimentation velocity Original Research Article Pages 296-306 John R Cann, Walter B Goad [13] Pressure effects in ultracentrifugation of interacting systems Original Research Article Pages 306-345 William F Harrington, Gerson Kegeles [14] Characterization of proteins by sedimentation equilibrium in the analytical ultracentrifuge Original Research Article Pages 346-441 David C Teller [15] Studies of protein ligand binding by gel permeation techniques Original Research Article Pages 441-455 Gary K Ackers [16] Determination of equilibrium constants by countercurrent distribution Original Research Article Pages 456-464 Gerson Kegeles [17] Rapid measurement of binding of ligands by rate of dialysis Original Research Article Pages 464-471 F.C Womack, S.P Colowick [18] Chromatography of proteins on hydroxyapatite Original Research Article Pages 471-479 Giorgio Bernardi [19] Rotating cylinder viscometers Original Research Article Pages 483-491 Elliott L Uhlenhopp, Bruno H Zimm [20] Solubility measurements Original Research Article Pages 491-497 Yasuhiko Nozaki [21] Ultraviolet difference spectroscopy—new techniques and applications Original Research Article Pages 497-525 John W Donovan [22] Spectrophotometric titration of the functional groups of proteins Original Research Article Pages 525-548 John W. Donovan [23] Difference infrared spectrophotometric titration of protein carboxyls Original Research Article Pages 548-557 Serge N Timasheff, H Susi, John A Rupley [24] Differential conductimetry Original Research Article Pages 557-590 Alkis J Sophianopoulos [25] Thermal titrimetry Original Research Article Pages 591-616 Mario A Marini, Charles J Martin [26] Negative stain electron microscopy of protein macromolecules Original Research Article Pages 617-672 Robert M Oliver [27] Circular dichroism and optical rotatory dispersion of proteins and polypeptides Original Research Article Pages 675-735 Alice J Adler, Norma J Greenfield, Gerald D Fasman [28] Difference optical rotatory dispersion and circular dichroism Original Research Article Pages 736-750 Jen Tsi Yang, Kue Hung Chau [29] Analysis of optical activity spectra of turbid biological suspensions Original Research Article Pages 751-767 Allan S Schneider [30] UV absorption and circular dichroism measurements on light scattering biological specimens; fluorescent cell and related large-angle light detection techniques Original Research Article Pages 767-796 Burton P Dorman, John E Hearst, Marcos F Maestre [31] Magneto optical rotation spectroscopy Original Research Article Pages 796-810 Victor E Shashoua [32] Differential spectrofluorometry Original Research Article Pages 811-822 Gerald D Fasman, Barkev Bablouzian [33] Nuclear magnetic resonance spectroscopy of proteins Original Research Article Pages 825-836 W.D Phillips [34] The prospects for carbon-13 nuclear magnetic resonance studies in enzymology Original Research Article Pages 836-911 Frank R.N Gurd, Philip Keim [35] Mössbauer spectroscopy Original Research Article Pages 912-941 Thomas H Moss [36] Automated edman degradation: The protein sequenator Original Research Article Pages 942-1010 Hugh D Niall Author index Pages 1011-1035 Subject index Pages 1036-1063
