Practical Immunology, Fourth Edition

Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialized immunologists who need to use an immunological technique outside their sphere of expertise. The book gives straightforward descriptions of the most common techniques that are referred to, but not always well explained, in the current literature. Tips for improving the techniques, troubleshooting and other handy bits of advice make it more than just a book of standard protocols. Where possible, the techniques are explained in such a way that the purchase of specialist kits is avoidable. For those who are a little rusty on their basic immunology the book also gives background information, making it the only "one stop shop" around. Practical Immunology tells you how to do the basic techniques that form the anatomy of experimental immunology. This latest edition has been fully revised to include the basic techniques of molecular biology, with a revised section on cytokines, an expanded section on clinical immunology and new techniques in epitope mapping and the study of cell adhersion molecules. Practical Immunology is an introductory text aimed at students and researchers who need a comprehensive overview of the methodology of immunology. Now in its fourth edition, Practical Immunology will appeal to students of immunology, graduate students embarking on bench science, and specialized immunologists who need to use a technique outside their sphere of expertise.The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice. Practical Immunology gives straightforward descriptions of the more commonly used techniques that are often covered, but not always clearly described, in the research literature. It explains how to perform these basic methodologies that form the anatomy of experimental immunology, and includes tips for improving the techniques, troubleshooting and other helpful suggestions. Where possible, the methods are explained in a way to ensure that the purchase of specialist kits is avoidable. For those who are a little out of practice on their basic immunology, the book also gives some background information, making it a highly useful 'one stop shop'.This latest edition has been fully revised to include basic techniques found in the appropriate chapters, with revised sections on cytokines, clinical immunology, new techniques in epitope mapping and the study of cell adhesion molecules.

Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise.

• The definitive lab "bench book".
  
• A one stop resource.
  
• Techniques explained from first principles.
  
• Basic forms of apparatus described in detail.
  
• Totally revised with new user friendly layout to aid use in the lab.
  
• Includes useful hints and tips.

Biochimica Clinica

...In summary, anyone who wishes to gain a good knowledge of Immunology can benefit from this book.

Content: Chapter 1 Isolation and Structure of Immunoglobulins (pages 1–39): Chapter 2 Monoclonal Antibodies: Production, Purification and Enzymatic Fragmentation (pages 40–70): Chapter 3 Antibody Interactions with Antigens (pages 71–114): Chapter 4 Antibodies as Probes (pages 115–162): Chapter 5 Immunoassay (pages 163–177): Chapter 6 Isolation of Cells (pages 179–202): Chapter 7 Phagocytosis, Complement and Antibody?dependent Cytotoxicity (pages 203–227): Chapter 8 Lymphocyte Structure (pages 228–260): Chapter 9 Lymphocyte Function (pages 261–300): Chapter 10 The Cytokines (pages 301–325): Chapter 11 Immunological Manipulations in vivo (pages 326–345): The relative solubility of proteins in pure water, ethanol or various salt solutions may be used as a basic fractionation technique.