Protocols for Adult Stem Cells

The study of adult stem cells has surged in recent years. Because they are responsible for the body’s natural ability to fight diseases, heal and recover, or fail and succumb to various maladies, it has become increasingly important to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. In __Protocols for Adult Stem Cells__, expert researchers present a variety of methods for studying five types of clinically-relevant mammalian stems cells: mammary, nerve, skeletal muscle, endothelial and mesenchymal. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Chapters include information that will assist researchers in obtaining, characterizing and studying these cells or adapting them to the stem cells of choice. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Critical and cutting edge, __Protocols for Adult Stem Cells__ is an essential guide which provides groundbreaking and novel techniques certain to redefine the field of stem cell biology. In Situ Analysis Of Cell Populations: Long-term Label-retaining Cells / Rodrigo Fernandez-gonzalez, Irineu Illa-bochaca, Dawne N. Shelton, Bryan E. Welm, Mary Helen Barcellos-hoff, Carlos Ortiz-de-solorzano -- Limiting-dilution Transplantation Assays In Mammary Stem Cell Studies / Irineu Illa-bochaca, Rodrigo Fernandez-gonzalez, Dawne N. Shelton, Bryan E. Welm, Carlos Ortiz-de-solorzano, Mary Helen Barcellos-hoff -- Use Of Stem Cell Markers In Dissociated Mammary Populations / Dawne N. Shelton, Rodrigo Fernandez-gonzalez, Irineu Illa-bochaca, Carlos Ortiz-de-solorzano, Mary Helen Barcellos-hoff, Bryan E. Welm. Isolation Of Adult Hippocampal Neural Progenitors / Joseph Peltier, Brandi K. Ormerod, David V. Schaffer -- In Vitro Culture And Analysis Of Adult Hippocampal Neural Progenitors / Joseph Peltier, Smita Agrawal, Matthew J. Robertson, David V. Schaffer -- In Vivo Analysis Of Engrafted Adult Hippocampal Neural Progenitors / Matthew J. Robertson, Joseph Peltier, David V. Schaffer -- Viral Packaging And Transduction Of Adult Hippocampal Neural Progenitors / Joseph Peltier, David V. Schaffer -- Culture Of Bone Marrow Mesenchymal Stem Cells On Engineered Matrix / Jennifer S. Park, Craig Hashi, Song Li. Preparation And Analysis Of Endothelial Progenitor Cells Associated With Angiogenesis / Steve Khachi, Yiqian Zhu, Guo-yuan Yang -- Preparation Of Adult Muscle Fiber-associated Stem/precursor Cells / Michael J. Conboy, Irina M. Conboy -- Immuno-analysis And Facs Sorting Of Adult Muscle Fiber-associated Stem/precursor Cells / Michael J. Conboy, Massimiliano Cerletti, Amy J. Wagers, Irina M. Conboy -- Altered Expression Of Nfkb In Ex Vivo Differentiated Dendritic Cells From The Aged Subjects: Implications In Immunotherapy / Anshu Agrawal. Preparation Of Collagen-coated Gels That Maximize In Vitro Myogenesis Of Stem Cells By Matching The Lateral Elasticity Of In Vivo Muscle / Tathagata Chaudhuri, Florian Rehfeldt, H. Lee Sweeney, Dennis E. Discher. Edited By Irina M. Conboy ... [et Al.]. Includes Bibliographical References And Index. Front Matter....Pages i-x Back Matter....Pages 1-28 ....Pages 29-47