Reconstituting the Cytoskeleton (ISSN Book 540)

This new volume of __Methods in Enzymology__ continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth.* Continues the legacy of this premier serial with quality chapters authored by leaders in the field * Covers cytoskeletal structure * Contains chapters with such topics as reconstitution of organelle transport and assaying microtubule nucleation by gamma-tubulin ring complex. Content: Series Page Page ii Copyright Page iv Contributors Pages xiii-xviii Preface: The Role of Reconstitution in Cytoskeleton Research Pages xix-xxiii Ronald D. Vale Chapter One - Actin Filament Dynamics Using Microfluidics Pages 3-17 Marie-France Carlier, Guillaume Romet-Lemonne, Antoine Jégou Chapter Two - Bacterial Actin-Like Proteins: Purification and Characterization of Self-Assembly Properties Pages 19-34 Natalie A. Petek, R. Dyche Mullins Chapter Three - Quantitative Analysis of Microtubule Self-assembly Kinetics and Tip Structure Pages 35-52 Louis S. Prahl, Brian T. Castle, Melissa K. Gardner, David J. Odde Chapter Four - Biochemical Reconstitution of the WAVE Regulatory Complex Pages 55-72 Baoyu Chen, Shae B. Padrick, Lisa Henry, Michael K. Rosen Chapter Five - Rotational Movement of Formins Evaluated by Using Single-Molecule Fluorescence Polarization Pages 73-94 Hiroaki Mizuno, Naoki Watanabe Chapter Six - Single-Molecule Studies of Actin Assembly and Disassembly Factors Pages 95-117 Benjamin A. Smith, Jeff Gelles, Bruce L. Goode Chapter Seven - Assaying Microtubule Nucleation by the γ-Tubulin Ring Complex Pages 119-130 Yuk-Kwan Choi, Robert Z. Qi Chapter Eight - Reconstituting Dynamic Microtubule Polymerization Regulation by TOG Domain Proteins Pages 131-148 Jawdat Al-Bassam Chapter Nine - Generation of Differentially Modified Microtubules Using In Vitro Enzymatic Approaches Pages 149-166 Annapurna Vemu, Christopher P. Garnham, Duck-Yeon Lee, Antonina Roll-Mecak Chapter Ten - Engineering Defined Motor Ensembles with DNA Origami Pages 169-188 Brian S. Goodman, Samara L. Reck-Peterson Chapter Eleven - Construction and Analyses of Elastically Coupled Multiple-Motor Systems Pages 189-204 Arthur Rogers, Pamela E. Constantinou, D. Kenneth Jamison, Jonathan W. Driver, Michael R. Diehl Chapter Twelve - Reconstitution of Cortical Dynein Function Pages 205-230 Sophie Roth, Liedewij Laan, Marileen Dogterom Chapter Thirteen - Reconstitution of Microtubule-Dependent Organelle Transport Pages 231-248 Pradeep Barak, Ashim Rai, Alok Kumar Dubey, Priyanka Rai, Roop Mallik Chapter Fourteen - Reconstituting the Motility of Isolated Intracellular Cargoes Pages 249-262 Adam G. Hendricks, Yale E. Goldman, Erika L.F. Holzbaur Chapter Fifteen - Reconstitution of Contractile Actomyosin Arrays Pages 265-282 Michael Murrell, Todd Thoresen, Margaret Gardel Chapter Sixteen - Directed Actin Assembly and Motility Pages 283-300 Rajaa Boujemaa-Paterski, Rémi Galland, Cristian Suarez, Christophe Guérin, Manuel Théry, Laurent Blanchoin Chapter Seventeen - In Vitro Reconstitution of Dynamic Microtubules Interacting with Actin Filament Networks Pages 301-320 Magdalena Preciado López, Florian Huber, Ilya Grigoriev, Michel O. Steinmetz, Anna Akhmanova, Marileen Dogterom, Gijsje H. Koenderink Chapter Eighteen - Measuring Kinetochore–Microtubule Interaction In Vitro Pages 321-337 Jonathan W. Driver, Andrew F. Powers, Krishna K. Sarangapani, Sue Biggins, Charles L. Asbury Chapter Nineteen - Micropattern-Guided Assembly of Overlapping Pairs of Dynamic Microtubules Pages 339-360 Franck J. Fourniol, Tai-De Li, Peter Bieling, R. Dyche Mullins, Daniel A. Fletcher, Thomas Surrey Chapter Twenty - WAVE Regulatory Complex Activation Pages 363-379 Peter J. Hume, Daniel Humphreys, Vassilis Koronakis Chapter Twenty-One - Dissecting Principles Governing Actin Assembly Using Yeast Extracts Pages 381-397 Alphée Michelot, David G. Drubin Chapter Twenty-Two - Xenopus Egg Cytoplasm with Intact Actin Pages 399-415 Christine M. Field, Phuong A. Nguyen, Keisuke Ishihara, Aaron C. Groen, Timothy J. Mitchison Chapter Twenty-Three - Glycogen-Supplemented Mitotic Cytosol for Analyzing Xenopus Egg Microtubule Organization Pages 417-433 Aaron C. Groen, Phuong A. Ngyuen, Christine M. Field, Keisuke Ishihara, Timothy J. Mitchison Chapter Twenty-Four - Spindle Assembly on Immobilized Chromatin Micropatterns Pages 435-448 Céline Pugieux, Serge Dmitrieff, Katarzyna Tarnawska, François Nédélec Author Index Pages 449-470 Subject Index Pages 471-484 This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cytoskeletal structure, including such topics as rotational movement of formins studied by fluorescence polarization microscopy, in vitro assembly assays for bacterial actin filaments, and modulators of microtubule plus end growth.

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers cytoskeletal structure
• Contains chapters with such topics as reconstitution of organelle transport and assaying microtubule nucleation by gamma-tubulin ring complex.