Signal Transduction Protocols

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, __Signal Transduction Protocols, Third Edition__ focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful __Methods in Molecular BiologyTM__ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, __Signal Transduction Protocols__ serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research. Refining Efficacy : Allosterism And Bias In G Protein-coupled Receptor Signaling / Louis M. Luttrell And Terry P. Kenakin -- Imaging-based Approaches To Understanding G Protein-coupled Receptor Signalling Complexes / Darlaine Petrin And Terence E. Hebert -- Improving Drug Discovery With Contextual Assays And Cellular Systems Analysis / John K. Westwick And Jane E. Lamerdin -- Rgs-insensitive Ga Subunits : Probes Of Ga Subtype-selective Signaling And Physiological Functions Of Rgs Proteins / Kuljeet Kaur ... [et Al.] -- Bioinformatic Approaches To Metabolic Pathways Analysis / Stuart Maudsley ... [et Al.] -- Studying Ligand Efficacy At G Protein-coupled Receptors Using Fret / Jean-pierre Vilardaga -- Using Bret To Detect Ligand-specific Conformational Changes In Preformed Signalling Complexes / Nicolas Audet And Graciela Pineyro -- Reconstitution Of G Protein-coupled Receptors Into A Model Bilayer System : Reconstituted High-density Lipoprotein Particles / Gisselle A. Velez-ruiz And Roger K. Sunahara -- Using Quantitative Bret To Assess G Protein-coupled Receptor Homo- And Heterodimerization / Lamia Achour .. [et Al.] -- Cell-surface Protein-protein Interaction Analysis With Time-resolved Receptor Oligomerization / Laetitia Comps-agrar ... [et Al.] -- Analysis Of Gpcr/ion Channel Interactions / Christophe Altier And Gerald W. Zamponi -- Multicolor Bifc Analysis Of G Protein Bg Complex Formation And Localization / Thomas R. Hynes -- Real-time Bret Assays To Measure G Protein/effector Interactions / Darlaine Petrin, Melanie Robitaille, And Terence E. Hebert -- Luminescent Biosensors For Real-time Monitoring Of Intracellular Camp / Brock F. Binkowski, Frank Fan, And Keith V. Wood -- Simultaneous Real-time Imaging Of Signal Oscillations Using Multiple Fluorescence-based Reporters / Lianne B. Dale And Stephen S. G. Ferguson -- Using Fret-based Reporters To Visualize Subcellular Dynamics Of Protein Kinase A Activity / Charlene Depry And Jin Zhang -- Genetically Encoded Fluorescent Reporters To Visualize Protein Kinase C Activation In Live Cells / Lisa L. Gallegos And Alexandra C. Newton -- Visualizing Receptor Endocytosis And Trafficking / Ali Salahpour And Larry S. Barak -- Investigating G Protein-coupled Receptor Endocytosis And Trafficking By Tir-fm / Guillermo A. Yudowski And Mark Von Zastrow -- Visualizing G Protein-coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy / Sudha K. Shenoy -- Detection And Characterization Of Receptor Interactions With Pdz Domains / Stefanie L. Ritter And Randy A. Hall -- Tandem Affinity Purification And Identification Of Heterotrimeric G Protein-associated Proteins / Syed M. Ahmed, Avais M. Daulat, And Stephane Angers -- Study Of G Protein-coupled Receptor/b-arrestin Interactions Within Endosomes Using Frap / Benjamin Aguila, May Simaan, And Stephane A. Laporte -- Disrupting Protein Complexes Using Tat-tagged Peptide Mimics / Shupeng Li .. [et Al.] -- Protein-fragment Complementation Assays For Large-scale Analysis, Functional Dissection And Dynamic Studies Of Protein-protein Interactions In Living Cells / Stephen W. Michnick .. [et Al.]. Edited By Louis M. Luttrell, Stephen S. G. Ferguson. Includes Bibliographical References And Index. Front Matter....Pages i-xiv Front Matter....Pages 1-1 Front Matter....Pages 3-35 Front Matter....Pages 37-60 Front Matter....Pages 61-73 Front Matter....Pages 75-98 Front Matter....Pages 99-130 Back Matter....Pages 131-131 ....Pages 133-148 It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, this book focuses on experimental approaches to better understand the complexity of signal transduction.