Single cell analysis : technologies and applications

The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.Content: Chapter 1 Single Molecule Fluorescence Monitoring in Eukaryotic Cells: Intranuclear Dynamics of Splicing Factors (pages 1–17): Ulrich KubitscheckChapter 2 Gene Classification and Quantitative Analysis of Gene Regulation in Bacteria Using Single Cell Atomic Force Microscopy and Single Molecule Force Spectroscopy (pages 19–37): Robert Ros and Nicole HansmeierChapter 3 Cellular Cryo?Electron Tomography (CET): Towards a Voyage to the Inner Space of Cells (pages 39–68): Juergen M. PlitzkoChapter 4 Single Cell Proteomics (pages 67–89): Norman J. Dovichi, Shen Hu, David Michels, Danqian Mao and Amy DambrowitzChapter 5 Protein Analysis of Single Cells in Microfluidic Format (pages 91–107): Alexandra Ros and Dominik GreifChapter 6 Single Cell Mass Spectrometry (pages 109–133): Elena V. Romanova, Stanislav S. Rubakhin, Eric B. Monroe and Jonathan V. SweedlerChapter 7 Single Cell Analysis for Quantitative Systems Biology (pages 135–160): Luke P. Lee and Dino Di CarloChapter 8 Optical Stretcher for Single Cells (pages 161–174): Karla Muller, Anatol Fritsch, Tobias Kiessling, Marc Grosseruschkamp and Josef A. KasChapter 9 Single Cell Immunology (pages 173–195): Ulrich Walter and Jan BuerChapter 10 Molecular Characterization of Rare Single Tumor Cells (pages 197–221): James F. LearyChapter 11 Single Cell Heterogeneity (pages 223–234): Edgar A. ArriagaChapter 12 Genome and Transcriptome Analysis of Single Tumor Cells (pages 235–249): Bernhard Polzer, Claudia H. Hartmann and Christoph A. Klein

“this Volume Presents An Excellent Overview Into The World Of Cell Adhesion. It Provides Important Details About The Main Players In All Aspects Of Cell Junctions.” –the Quarterly Review Of Biology, September 2009

eagerly Awaited, this Comprehensive Book On a Topic Of Fundamental Importance In Our Understanding Of Cancer Begins With An Overview Of Cellular Junctions, Before Covering In Detail Cell-matrix Junctions, cell-cell Junctions As Well As cell-matrix And Cell-cell Adhesion.

this Reference Work is Of High Interest To Cell And Molecular Biologists, Cancer Researchers, Oncologists, Biochemists, Pharmaceutists And Those Working In The Pharmaceutical Industry.

doody Review Services

reviewer:bruce A. Fenderson, Phd(thomas Jefferson University)
description:cell Junctions Are Clusters Of Proteins That Provide Adhesive Contacts Between Cells And Their Environment. These Receptors And Transmembrane Signaling Molecules Play A Key Role In Regulating Morphogenesis, Differentiation, And Wound Healing. As Noted By The Editors, Cell Junctions Not Only Mediate Physical Interactions, But Also Couple Cell Contact To Signaling Pathways That Influence Cell Shape, Cell Cycle Progression, And Gene Expression. This Book Reviews The Literature On Cell Junctions In Development And Disease, With 14 Concise Chapters On Topics Ranging From Cell Matrix Adhesion In Three Dimensions To Cadherin Trafficking And Junction Dynamics. The Emphasis Is On Cell Biology And Protein Biochemistry.
purpose:the Purpose Is To Assemble Important Contributions From Some Of The World's Leading Experts Into One Book. The Authors Hope This Book Will Provide A Resource That Is Valuable To The New Student Of Cell Junctions, And To Experienced Scientists In The Field, And To Those Educators Who Require A Comprehensive Assembly Of Overviews In The Discipline Of Cell Contact And Adhesion. The Authors Are Experts In This Exciting, Interdisciplinary Field.
audience:the Book Is Written For Basic Science And Clinical Researchers (graduate Students, Postdoctoral Fellows, And Scientists) Interested In Cell/developmental Biology, Biochemistry, Biophysics, And Bioengineering. Oncologists And Those Working In The Pharmaceutical Industry Will Also Appreciate This In-depth Overview Of The Cell Adhesion Literature.
features:the Authors Wish To Understand How Cell Junction Signaling Changes During Development And How Defects In Cell Junction Signaling Contribute To Disease. The Book Is Divided Into Three Major Sections: L) Cell-matrix Junctions With An Emphasis On Integrins; 2) Cell-cell Junctions With An Emphasis On Cadherins; And 3) Cross-talk Between These Two Types Of Cell Surface Contacts. This Is Not A Protocol Book, But An Up-to-date Review Of The Literature. Each Chapter Begins With An Introduction And Concludes With A Brief Summary Or Discussion Of Future Research Prospects. Results Of Gene Knockout Studies Are Provided For Key Proteins. The Line Drawings And Photomicrographs Are Informative And Well Done, And Several Figures Are Printed In Full Color. The Authors Provide Complete References To Facilitate Scholarly Research.
assessment:the Chapters Are Readable Accounts Of A Very Rich And Complicated Literature. The Authors Illustrate How Cell Surface Proteins Integrate Signals To Coordinate Complex Processes Such As Cell Migration And Morphogenesis. The Chapters Are Well Written And The Book Is Carefully Edited. The Excellent Preface Helps Place The Individual Chapters Into Perspective. The Authors Focus On Important Topics And Provide A Snapshot Of Current Research Findings. An Important Feature Of This Book Is The Focus On Cell Junctions In Health And Disease (i.e., Clinical Correlations). This Is A Welcome Addition To The Literature That Will Be Valued By All Students Of Cell Adhesion And Morphogenesis.

Since The Discovery Of The Gene For Green Fluorescent Protein (gfp), Derived From Jellyfish, This Protein That Emits A Green Glow Has Initiated A Revolution In Molecular Biosciences. With This Tool, It Is Now Possible To Visualize Nearly Any Protein Of Interest In Any Cell Or Tissue Of Any Species. Discovery Of Green Fluorescent Protein / Osamu Shimomura -- Photons For Reporting Molecular Events: Green Fluorescent Protein And Four Luciferase Systems / J. Woodland Hastings And James G. Morin -- Biochemcical And Physical Properties Of Green Fluorescent Protein / William W. Ward -- The Three-dimensional Structure Of Green Fluorescent Protein And Its Implications For Function And Design / George N. Phillips Jr -- Molecular Biology And Mutation Of Green Fluorescent Protein / David A. Zacharias And Roger Y. Tsien -- Discovery And Properties Of Gfp-like Proteins From Non-bioluminescent Anthozoa / Konstantin A. Lukyanov ... [et Al.]. Evolution Of Function And Color In Gfp-like Proteins / Mikhail V. Matz, Yulii A. Labas, And Juan Ugalde -- The Uses Of Green Fluorescent Protein In Prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, And Stanley Falkow -- The Uses Of Green Fluorescent Protein In Yeasts / Amy L. Hitchcock, Jason A. Kahana, And Pamela A. Silver -- Uses Of Gfp In Caenorhabditis Elegans / Oliver Hobert And Paula Loria -- Green Fluorescent Protein Applications In Drosophila / Tulle Hazelrigg And Jennifer H. Mansfield -- The Uses Of Green Fluorescent Protein In Plants / Jim Haseloff And Kirby R. Siemering -- Uses Of Gfp In Transgenic Vertebrates / Sean Megason ... [et Al.]. The Uses Of Green Fluorescent Protein In Mammalian Cells / Theresa H. Ward And Jennifer Lippincott-schwartz -- Practical Consideration For Use Of Reef Coral Fluorescent Proteins In Mammalian Cells: Applications In Fluorescent Microscopy And Flow Cytometry / Yu Fang ... [et Al.] -- Pharmaceutical Applications Of Gfp And Rcfp / Nicola Bevan And Stephen Rees -- Reassembled Gfp: Detecting Protein-protein Interactions And Protein Expression Patterns / Thomas J. Magliery And Lynne Regan. Edited By Martin Chalfie And Steven R. Kain. Includes Bibliographical References And Index. Also Available In An Electronic Version. Mode Of Access: World Wide Web. Discovery of green fluorescent protein / Osamu Shimomura Photons for reporting molecular events: green fluorescent protein and four luciferase systems / J. Woodland Hastings and James G. Morin Biochemical and physical properties of green fluorescent protein / William W. Ward The three-dimensional structure of green fluorescent protein and its implications for function and design / George N. Phillips, Jr Molecular biology and mutation of green fluorescent protein / David A. Zacharias, Roger Y. Tsien Discovery and properties of GFP-like proteins from nonbioluminescent anthozoa / Konstantin A. Lukyanov ... [et al.] Evolution of function and color in GFP-like proteins / Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde The uses of green fluorescent protein in prokaryotes / Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow The uses of green fluorescent protein in yeasts / Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver Uses of GFP in Caenorhabditis elegans / Oliver Hobert and Paula Loria Green fluorescent protein applications in drosophila / Tulle Hazelrigg and Jennifer H. Mansfield The uses of green fluorescent protein in plants / Jim Haseloff and Kirby R. Siemering Uses of GFP in transgenic vertebrates / Sean Magason ... [et al.] The uses of green fluorescent protein in mammalian cells / Theresa H. Ward and Jennifer Lippincott-Schwartz Practical considerations for use of reef coral fluorescent proteins in mammalian cells: applications in fluorescent microscopy and flow cytometry / Yu Fang ... [et al.] Pharmaceutical applications of GFP and RCFP / Nicola Bevan and Stephen Rees Reassembled GFP: detecting protein-protein interactions and protein expression patterns / Thomas J. Magliery and Lynne Regan. Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection. Yanagida (Osaka University Graduate School of Frontier Biosciences) and Ishii (Core Research for Evolutional Science and Technology, Japan Science and Technology Agency) gather pioneers in the field of single molecule science to show how to set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, contributors consider molecular motors and mechanical properties, then move on to applications. Detailed discussions of studies on protein enzymes, ribozymes, and nucleic acids are also included. Some chapter topics include imaging and molecular motors, signal transduction across the plasma membrane, single-molecule tracking of quantum dot liganded epidermal growth factor, single- molecule FRET studies of helicases and Holliday junctions, and high- speed atomic force microscopy for nano-visualization of biomolecular processes. B & w and color images and illustrations are included. Annotation ©2009 Book News, Inc., Portland, OR --From booknews.com Nanotechnology is the key technology of the 21st century. The possibility to exploit the structures and processes of biomolecules for novel functional materials, biosensors, bioelectronics and medical applications has created the rapidly growing field of nanobiotechnology. Designed as a broad survey of the field, this book combines contributions from bioorganic and bioinorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of current and future developments. It is divided into four main sections: interphase systems; protein based nanostructures; DNA based nanostructures; nanoanalytics. Each chapter describes in detail currently available methods and contains numerous references to the primary literature, making this the perfect "field guide" for chemists, biologists and materials scientists who want to explore the fascinating world of nanobiotechnology

advances In Instrumentation Over The Last Few Years Have Established Single Molecule Measurements As A Major Method In Many Biological Laboratories. Exploiting The Full Power Of Such Measurements Is Dependent On A Good Understanding Of Single Molecule Behavior And Enzymology.

in This First Comprehensive Resource On The Topic, One Of The World's Most Distinguished Single Molecule Scientists Has Put Together A Team Of Pioneers In The Field To Show How To Both Set Up And Interpret A Single Molecule Experiment. Following An Introduction To Single Molecule Measurements And Enzymology, The Authors Consider Molecular Motors And Mechanical Properties Before Moving On To The Applications Themselves. Detailed Discussions Of Studies On Protein Enzymes, Ribozymes And Nucleic Acids Are Also Included.

this Book Is Indispensable Reading For A Growing Number Of Scientists.

This first modern book on the topic gives a broad overview on current technology and application areas for single cell approaches in life sciences. Identification and imaging of single cells which is the most widely applied single cell technology, is described in the opening section, including fluorescence, electron tomography and atomic force techniques. The core section of the book covers a wide range of technologies for the handling, manipulation and constituent analysis of individual cells. The final section is dedicated to case studies and specific applications, with examples ranging from cell biology and genetics to molecular medicine. "This volume presents an excellent overview into the world of cell adhesion. It provides important details about the main players in all aspects of cell junctions."--The Quarterly Review of Biology, September 2009. Eagerly awaited, this comprehensive book on a topic of fundamental importance in our understanding of cancer begins with an overview of cellular junctions, before covering in detail cell-matrix junctions, cell-cell junctions as well as cell-matrix and cell-cell adhesion. This reference work is of high interest to cell and molecular biologists, cancer researchers, oncologists, bioche. "Green fluorescent protein (GFP), a biological marker cloned from jellyfish, has emerged from virtual obscurity to the forefront of biomolecular research in just a few years. GFP and related fluorescent proteins (FPs) have been intensely studied - the subjects of hundreds of in vivo projects worldwide. These studies have resulted in new applications and published articles, emerging at a breathtaking rate. The success of FPs and their many uses promise to make them some of the most powerful tools in biotechnology, pharmaceutical science, and molecular and cellular biology." This second volume on a burgeoning field retains the proven concept of the spectacularly successful first one, extending and supplementing it. Individual sections are each dedicated to nanoparticles, nanostructures and patterns, nanodevices and machines, and nanoanalytics. Essential reading for an entire generation of scientists, this authoritative survey defines one of the most important new scientific fields to have emerged for many decades. This useful field guide for chemists, biologists and materials scientists is a broad survey of the field which combines contributions from bioorganic and bio-inorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of future developments New technologies have always been a major driving force in medical device technology [1], and it is largely due to the high economical and social value of modern medical devices that new materials and processes are incorporated at a very early stage into new products. The first comprehensive book on this topic of fundamental importance in our understanding of cancer begins with an overview of cellular junctions, before covering cell-matrix junctions, cell-cell junctions and cell-matrix and cell-cell adhesion in separate sections In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment.