Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in __Single Molecule Techniques: Methods and Protocols__ are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of __Single Molecule Techniques: Methods and Protocols__ is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful __Methods in Molecular BiologyTM__ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, __Single Molecule Techniques: Methods and Protocols__ serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology. Introduction to optical tweezers : background, system designs, and commercial solutions -- Joost van Mameren, Gijs J. L. Wuite, and Iddo Heller Optical trapping and unfolding of RNA -- Katherine H. White and Koen Visscher DNA unzipping and force measurements with a dual optical trap -- Ismaıl Cisse, Pierre Mangeol, and Ulrich Bockelmann Probing the force generation and stepping behavior of cytoplasmic dynein -- Arne Gennerich and Samara L. Reck-Peterson Brief introduction to single-molecule fluorescence methods -- Siet M. J. L. van den Wildenberg, Bram Prevo, and Erwin J. G. Peterman Fluorescent labeling of proteins -- Mauro Modesti Fluorescence imaging of single kinesin motors on immobilized microtubules -- Till Korten ... [ et al.] Exploring protein superstructures and dynamics in live bacterial cells using single-molecule and superresolution imaging -- Julie S. Biteen, Lucy Shapiro, and W.E. Moerner Fluorescence microscopy of nanochannel-confined DNA -- Fredrik Persson, Fredrik Westerlund, and Jonas O. Tegenfeldt Fluorescence correlation spectroscopy -- Patrick Ferrand, Jerome Wenger, and Herve Rigneault Introduction to atomic force microscopy -- Pedro J. de Pablo Sample preparation for SFM imaging of DNA, proteins, and DNA-protein complexes -- Dejan Ristic, Humberto Sanchez, and Claire Wyman Single-molecule protein unfolding and refolding using atomic force microscopy -- Thomas Bornschlogl and Matthias Rief How to perform a nanoindentation experiment on a virus -- Wouter H. Roos Magnetic tweezers for single-molecule manipulation -- Yeonee Seol and Keir C. Neuman Probing DNA topology using tethered particle motion -- David Dunlap ... [et al.]. Front Matter....Pages i-x Back Matter....Pages 1-20 ....Pages 21-43
