Spin Labeling : Theory and Applications

We present this special topics volume on an area which has not received thorough coverage for over 12 years. Spin Labeling: Theory and Applications represents a complete update on new theoretical aspects and applications of the spin-label method. In the "line-shape theory" sections, we are especially pleased to include an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations. Barney Bales discusses inhomogeneous broadening phenomena in detail. Several developments in techniques and interpretation in saturation transfer spectroscopy have appeared since the publica­ tion of Spin Labeling II: Theory and Applications (L. J. Berliner, ed., Academic Press, 1979). We have included an up-to-date chapter on spin-label applications by M. A. Hemminga and P. A. de Jager. By incorporating 15N and deuterium into nitroxide spin labels, several unique advantages are derived in line-shape analysis. Albert Beth and Bruce Robinson have contributed a detailed chapter on the analysis of these labels in the slow-motion regime while Jane Park and Wolfgang Trommer present the advantages for specific biochemical examples in our "applications" section. Derek Marsh's contri­ bution on spin-label spectral analysis may be regarded as a summary chapter which touches on several of the detailed spectral analysis methods described in the earlier chapters. We present this special topics volume on an area which has not received thorough coverage for over 12 years. Spin Labeling: Theory and Applications represents a complete update on new theoretical aspects and applications of the spin-label method. In the "line-shape theory" sections, we are especially pleased to include an IBM-compatible diskette supplied by David Schneider and Jack Freed which contains fast, accurate, ready-to-use software for slow-motion simulations. Barney Bales discusses inhomogeneous broadening phenomena in detail. Several developments in techniques and interpretation in saturation transfer spectroscopy have appeared since the publicaƯ tion of Spin Labeling II: Theory and Applications (L.J. Berliner, ed., Academic Press, 1979). We have included an up-to-date chapter on spin-label applications by M.A. Hemminga and P.A. de Jager. By incorporating 15N and deuterium into nitroxide spin labels, several unique advantages are derived in line-shape analysis. Albert Beth and Bruce Robinson have contributed a detailed chapter on the analysis of these labels in the slow-motion regime while Jane Park and Wolfgang Trommer present the advantages for specific biochemical examples in our "applications" section. Derek Marsh's contriƯ bution on spin-label spectral analysis may be regarded as a summary chapter which touches on several of the detailed spectral analysis methods described in the earlier chapters Front Matter....Pages i-xix Calculating Slow Motional Magnetic Resonance Spectra....Pages 1-76 Inhomogeneously Broadened Spin-Label Spectra....Pages 77-130 Saturation Transfer Spectroscopy of Spin Labels....Pages 131-178 Nitrogen-15 and Deuterium Substituted Spin Labels for Studies of Very Slow Rotational Motion....Pages 179-253 Experimental Methods in Spin-Label Spectral Analysis....Pages 255-303 Electron-Electron Double Resonance....Pages 305-307 Resolved Electron-Electron Spin-Spin Splittings in EPR Spectra....Pages 339-397 Spin-Label Oximetry....Pages 399-425 Chemistry of Spin-Labeled Amino Acids and Peptides....Pages 427-488 Nitroxide Radical Adducts in Biology: Chemistry, Applications, and Pitfalls....Pages 489-546 Advantages of 15 N and Deuterium Spin Probes for Biomedical Electron Paramagnetic Resonance Investigations....Pages 547-595 Magnetic Resonance Study of the Combining Site Structure of a Monoclonal Anti-Spin-Label Antibody....Pages 597-613 Approaches to the Chemical Synthesis of 15 N and Deuterium Substituted Spin Labels....Pages 615-634 Back Matter....Pages 635-650