Synaptosomes 1st Ed. 2018

This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses.After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In __Neuromethods__ series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, __Synaptosomes__ is a valuable resource for any researcher interested in learning more about this developing field. Front Matter ....Pages i-xv Front Matter ....Pages 1-1 Introduction to Synaptosomes (Kathryn M. Murphy)....Pages 3-6 Front Matter ....Pages 7-7 Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications (Herbert Zimmermann)....Pages 9-26 Synaptosome Preparations: Which Procedure Should I Use? (Peter R. Dunkley, Phillip J. Robinson)....Pages 27-53 Front Matter ....Pages 55-55 Protocol for a High-Throughput Semiautomated Preparation for Filtered Synaptoneurosomes (Justin L. Balsor, Kathryn M. Murphy)....Pages 57-73 Subcellular Fractionation of Brain Tissue from Small Tissue Explants (Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, Maria Florencia Angelo)....Pages 75-84 Preparation of P2 or Percoll-Purified Synaptosomes from Mammalian Brain Tissue (Jing Xue, Annie Quan, Phillip J. Robinson)....Pages 85-105 Front Matter ....Pages 107-107 Synaptosomal Bioenergetics and Glutamate Release (David G. Nicholls)....Pages 109-129 Synaptosome Bioenergetics and Calcium Handling: Aging Response (Silvia Lores-Arnaiz, Georgina Rodríguez de Lores Arnaiz, Analía G. Karadayian, Juanita Bustamante)....Pages 131-151 Isolation of Highly Purified and Functionally Active Synaptic Mitochondria (Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin et al.)....Pages 153-163 Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes (Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson et al.)....Pages 165-191 Front Matter ....Pages 193-193 Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein’s Effect on Neurotransmitter Release (Marianne Cilluffo, Theodore A. Sarafian, Joseph B. Watson)....Pages 195-208 Porosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion, and Neurotransmitter Release (Bhanu P. Jena)....Pages 209-225 SMPL Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses (Riana Lo Bu, Jeffrey Clark, Thomas DiChiara, Stephen G. Sligar, William L. Klein)....Pages 227-250 Front Matter ....Pages 251-251 Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model (Amrit S. Boese, Anna Majer, Stephanie A. Booth)....Pages 253-267 Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex (Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steven J. Mancini, Kathryn M. Murphy)....Pages 269-286 The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders (Nadeem Murtaza, Vickie Kwan, Biren Dave, Karun K. Singh)....Pages 287-296 Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases (Nadia O. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, Martin Darvas)....Pages 297-310 Back Matter ....Pages 311-312 This Volume Describes Techniques To Make Synaptosomes, And Discusses Numerous Ways That Synaptosomes Are Used To Study And Answer Diverse Questions About Synapses. After Examining The History That Led To Making Synaptosomes, The Chapters Delved Into Its Important As A Tool To Explore Synaptic Connections. Some Of The Topics Covered In This Book Are: Synaptosome Bioenergetics And Glutamate Release; Affinity Proteomics For Interactome And Phosphoproteome Screening In Synaptosomes; Isolation Of Highly Purified And Functionally Active Synaptic Mitochondria; And Profiling Of Microrna And Protein From Purified Synaptoneurosomes In A Neurodegenerative Disease Model. In Neuromethods Series Style, Chapters Include The Kind Of Detail And Key Advice From The Specialists Needed To Get Successful Results In Your Laboratory. Cutting-edge And Thorough, Synaptosomes Is A Valuable Resource For Any Researcher Interested In Learning More About This Developing Field.