This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful __Methods in Molecular Biology__ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, __Wheat Biotechnology: Methods and Protocols__ serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world. Front Matter ....Pages i-xi Front Matter ....Pages 1-1 Enabling Molecular Technologies for Trait Improvement in Wheat (Prem L. Bhalla, Akanksha Sharma, Mohan B. Singh)....Pages 3-24 What Will Be the Benefits of Biotech Wheat for European Agriculture? (Agnès E. Ricroch)....Pages 25-35 Overview of the Wheat Genetic Transformation and Breeding Status in China (Jiapeng Han, Xiaofen Yu, Junli Chang, Guangxiao Yang, Guangyuan He)....Pages 37-60 Wheat Improvement in India: Present and Future (Sonia Goel, Kalpana Singh, N. K. Singh)....Pages 61-82 Overview of Methods for Assessing Salinity and Drought Tolerance of Transgenic Wheat Lines (Rohit Joshi, Khalid Anwar, Priyanka Das, Sneh L. Singla-Pareek, Ashwani Pareek)....Pages 83-95 Allergenicity Assessment of Transgenic Wheat Lines In Silico (Ankita Mishra, Naveen Arora)....Pages 97-111 Agribusiness Perspectives on Transgenic Wheat (Bill Malcolm)....Pages 113-126 Front Matter ....Pages 127-127 Agrobacterium-Mediated Transformation of Wheat Using Immature Embryos (Goetz Hensel, Cornelia Marthe, Jochen Kumlehn)....Pages 129-139 Biolistic Transformation of Wheat (Caroline Tassy, Pierre Barret)....Pages 141-152 Wheat Genetic Transformation Using Mature Embryos as Explants (Harsh Chauhan, Paramjit Khurana)....Pages 153-167 Targeted Mutagenesis in Hexaploid Bread Wheat Using the TALEN and CRISPR/Cas Systems (Yanpeng Wang, Yuan Zong, Caixia Gao)....Pages 169-185 Design and Assembly of CRISPR/Cas9 Reagents for Gene Knockout, Targeted Insertion, and Replacement in Wheat (Tomáš Čermák, Shaun J. Curtin)....Pages 187-212 Doubled Haploid Transgenic Wheat Lines by Microspore Transformation (Sachin Rustgi, Nii O. Ankrah, Rhoda A. T. Brew-Appiah, Yue Sun, Weiguo Liu, Diter von Wettstein)....Pages 213-234 Doubled Haploid Laboratory Protocol for Wheat Using Wheat–Maize Wide Hybridization (Meenakshi Santra, Hong Wang, Scott Seifert, Scott Haley)....Pages 235-249 Real-Time PCR for the Detection of Precise Transgene Copy Number in Wheat (Angelica Giancaspro, Agata Gadaleta, Antonio Blanco)....Pages 251-257 Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection (Litao Yang, Sheng Quan, Dabing Zhang)....Pages 259-268 Phenotypic Characterization of Transgenic Wheat Lines Against Fungal Pathogens Puccinia triticina and Fusarium graminearum (Jagdeep Kaur, Dilip Shah, John Fellers)....Pages 269-276 Databases for Wheat Genomics and Crop Improvement (Yuxuan Yuan, Armin Scheben, Chon-Kit Kenneth Chan, David Edwards)....Pages 277-291 High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives (Amanda J. Burridge, Mark O. Winfield, Alexandra M. Allen, Paul A. Wilkinson, Gary L. A. Barker, Jane Coghill et al.)....Pages 293-306 Back Matter ....Pages 307-309
