Genetic Engineering : Principles and Methods

__Jane K. Setlow__, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York. Front Matter....Pages i-xvi Cleavage-Site Motifs in Protein Targeting Sequences....Pages 1-11 Complications of RNA Heterogeneity for the Engineering of Virus Vaccines and Antiviral Agents....Pages 13-31 The Quaternary Structures of SV40 Large T Antigen and Tumor Suppressor p53: Analysis by Gel Electrophoresis....Pages 33-48 Assembly of Antibodies and Mutagenized Variants in Transgenic Plants and Plant Cell Cultures....Pages 49-64 Maize Endosperm Tissue as an Endoreduplication System....Pages 65-88 Study of Chlorate-Resistant Mutants of Arabidopsis: Insights into Nitrate Assimilation and Ion Metabolism of Plants....Pages 89-98 Approaches and Progress in the Molecular Cloning of Plant Disease Resistance Genes....Pages 99-124 Is GRP78 a Sensor of Cellular Secretory Activity?....Pages 125-137 The Molecular Biology of Pathogenesis in Ustilago Maydis ....Pages 139-162 Molecular Design of Oligomeric Channel Proteins....Pages 163-184 Regulation of Gene Expression by Thyroid Hormones and Retinoic Acid....Pages 185-209 RNA Trans -Splicing....Pages 211-229 Structural Constraints on Residue Substitution....Pages 231-249 Molecular and Functional Analysis of the a Mating Type Genes of Coprinus Cinereus ....Pages 251-268 Physical Mapping of Human Chromosomes....Pages 269-278 Back Matter....Pages 279-287 The current volume covers human gene therapy, improving the nutritional value of maize, restriction-modification enzymes, and eight other subjects