Genetic Engineering : Principles and Methods

This volume is the first of a series concerning a new technology which is revolutionizing the study of biology, perhaps as profoundly as the discovery of the gene. As pointed out in the introductory chapter, we look forward to the future impact of the technology, but we cannot see where it might take us. The purpose of these volumes is to follow closely the explosion of new techniques and information that is occurring as a result of the newly-acquired ability to make particular kinds of precise cuts in DNA molecules. Thus we are particularly committed to rapid publication.The Editor is most grateful to June Martino, who not only did all the final processing of the manuscripts, but also caught some of the Editor's mistakes as well as some of the authors'. vii CONTENTS DNA METHYlA. Front Matter....Pages i-ix DNA Methylases....Pages 1-11 Advances in Direct Gene Transfer into Cereals....Pages 13-31 The Copy Number Control System of the 2μm Circle Plasmid of Saccharomyces Cerevisiae....Pages 33-48 The Application of Antisense RNA Technology to Plants....Pages 49-63 The Pathogenesis-Related Proteins of Plants....Pages 65-109 The Molecular Genetics of Plasmid Partition: Special Vector Systems for the Analysis of Plasmid Partition....Pages 111-125 DNA-Mediated Transformation of Phytopathogenic Fungi....Pages 127-143 Fate of Foreign DNA Introduced to Plant Cells....Pages 145-157 Generation of cDNA Probes by Reverse Translation of Amino Acid Sequence....Pages 159-170 Molecular Genetics of Self-Incompatibility in Flowering Plants....Pages 171-181 Pulsed-Field Gel Electrophoresis....Pages 183-227 Back Matter....Pages 229-235