Guide to Protein Purification, 2nd Edition

The 2e of this classic __Guide to Protein Purification__ provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This ''Guide to'' gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. * Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology * Assembles chapters on both common and less common relevant techniques * Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process Content: Series Editors Pages i-iii Copyright Page Page iv Contents Pages v-xvii Contributors to Volume 463 Pages xix-xxiv Preface Pages xxv-xxvi Richard R. Burgess, Murray P. Deutscher Volume in Series Pages xxvii-liii Preface to Chapter 1 Page liv Murray P. Deutscher Chapter 1 Why Purify Enzymes? Pages 3-6 Arthur Kornberg Chapter 2 Strategies and Considerations for Protein Purifications Pages 9-19 Stuart Linn Chapter 3 Use of Bioinformatics in Planning a Protein Purification Pages 21-28 Richard R. Burgess Chapter 4 Preparing a Purification Summary Table Pages 29-34 Richard R. Burgess Chapter 5 Setting Up a Laboratory Pages 37-42 Murray P. Deutscher Chapter 6 Buffers: Principles and Practice Pages 43-56 Vincent S. Stoll, John S. Blanchard Chapter 7 Measurement of Enzyme Activity Pages 57-71 T.K. Harris, M.M. Keshwani Chapter 8 Quantitation of Protein Pages 73-95 James E. Noble, Marc J.A. Bailey Chapter 9 Concentration of Proteins and Removal of Solutes Pages 97-120 David R.H. Evans, Jonathan K. Romero, Matthew Westoby Chapter 10 Maintaining Protein Stability Pages 121-127 Murray P. Deutscher Chapter 11 Selecting an Appropriate Method for Expressing a Recombinant Protein Pages 131-147 William H. Brondyk Chapter 12 Bacterial Systems for Production of Heterologous Proteins Pages 149-168 Sarah Zerbs, Ashley M. Frank, Frank R. Collart Chapter 13 Expression in the Yeast Pichia pastoris Pages 169-189 James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden, Thomas Chappell Chapter 14 Baculovirus–Insect Cell Expression Systems Pages 191-222 Donald L. Jarvis Chapter 15 Recombinant Protein Production by Transient Gene Transfer into Mammalian Cells Pages 223-238 Sabine Geisse, Cornelia Fux Chapter 16 Tagging for Protein Expression Pages 239-258 Arun Malhotra Chapter 17 Refolding Solubilized Inclusion Body Proteins Pages 259-282 Richard R. Burgess Chapter 18 Advances in Preparation of Biological Extracts for Protein Purification Pages 285-303 Anthony C. Grabski Chapter 19 Isolation of Subcellular Organelles and Structures Pages 305-328 Uwe Michelsen, Jörg von Hagen Chapter 20 Protein Precipitation Techniques Pages 331-342 Richard R. Burgess Chapter 21 Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins Pages 343-345 Murray P. Deutscher Chapter 22 Ion-Exchange Chromatography Pages 349-371 Alois Jungbauer, Rainer Hahn Chapter 23 Gel Filtration Pages 373-385 Earle Stellwagen Chapter 24 Protein Chromatography on Hydroxyapatite Columns Pages 387-404 Larry J. Cummings, Mark A. Snyder, Kimberly Brisack Chapter 25 Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications Pages 405-414 Justin T. McCue Chapter 26 Affinity Chromatography: General Methods Pages 417-438 Marjeta Urh, Dan Simpson, Kate Zhao Chapter 27 Immobilized-Metal Affinity Chromatography (IMAC): A Review Pages 439-473 Helena Block, Barbara Maertens, Anne Spriestersbach, Nicole Brinker, Jan Kubicek, Roland Fabis, Jörg Labahn, Frank Schäfer Chapter 28 Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography Pages 475-494 Nancy E. Thompson, Katherine M. Foley, Elizabeth S. Stalder, Richard R. Burgess Chapter 29 One-Dimensional Gel Electrophoresis Pages 497-513 David E. Garfin Chapter 30 Isoelectric Focusing and Two-Dimensional Gel Electrophoresis Pages 515-540 David B. Friedman, Sjouke Hoving, Reiner Westermeier Chapter 31 Protein Gel Staining Methods: An Introduction and Overview Pages 541-563 Thomas H. Steinberg Chapter 32 Elution of Proteins from Gels Pages 565-572 Richard R. Burgess Chapter 33 Performing and Optimizing Western Blots with an Emphasis on Chemiluminescent Detection Pages 573-599 Alice Alegria-Schaffer, Andrew Lodge, Krishna Vattem Chapter 34 Detergents: An Overview Pages 603-617 Dirk Linke Chapter 35 Purification of Membrane Proteins Pages 619-629 Sue-Hwa Lin, Guido Guidotti Chapter 36 Purification of Recombinant G-Protein-Coupled Receptors Pages 631-645 Reinhard Grisshammer Chapter 37 Cell-Free Translation of Integral Membrane Proteins into Unilamelar Liposomes Pages 647-673 Michael A. Goren, Akira Nozawa, Shin-ichi Makino, Russell L. Wrobel, Brian G. Fox Chapter 38 Determination of Protein Purity Pages 677-689 David G. Rhodes, Thomas M. Laue Chapter 39 Determination of Size, Molecular Weight, and Presence of Subunits Pages 691-723 David G. Rhodes, Robert E. Bossio, Thomas M. Laue Chapter 40 Identification and Quantification of Protein Posttranslational Modifications Pages 725-763 Adam R. Farley, Andrew J. Link Chapter 41 Parallel Methods for Expression and Purification Pages 767-785 Scott A. Lesley Chapter 42 Techniques to Isolate O 2 -Sensitive Proteins: [4Fe–4S]-FNR as an Example Pages 787-805 Aixin Yan, Patricia J. Kiley Chapter 43 Rethinking Your Purification Procedure Pages 809-811 Murray P. Deutscher Chapter 44 Important but Little Known (or Forgotten) Artifacts in Protein Biochemistry Pages 813-820 Richard R. Burgess Author Index Pages 821-833 Subject Index Pages 835-851

The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today.

• Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology
• Assembles chapters on both common and less common relevant techniques
• Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

"The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process." The 2eof this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less-common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This'Guide to'gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process Examines the methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology. This guide relates how these methods connect to the explosive advancements in the field. It gives practical advice in order to avoid mistakes in choosing a method and presents a comprehensive overview of the field.