Lymphangiogenesis + Ereference: Methods And Protocols

This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, __Lymphangiogenesis: Methods and Protocols__ is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field. Front Matter ....Pages i-xii Three-Dimensional Visualization of the Lymphatic Vasculature (Cathrin Dierkes, Aaron Scherzinger, Friedemann Kiefer)....Pages 1-18 Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels (Kelly L. Betterman, Natasha L. Harvey)....Pages 19-35 Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice (Ines Martinez-Corral, Taija Makinen)....Pages 37-53 Visualization and Tools for Analysis of Zebrafish Lymphatic Development (Kazuhide S. Okuda, Sungmin Baek, Benjamin M. Hogan)....Pages 55-70 Characterization of Zebrafish Facial Lymphatics (Tiffany C. Y. Eng, Jonathan W. Astin)....Pages 71-83 Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development (Xin Geng, R. Sathish Srinivasan)....Pages 85-96 Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function (Amélie Sabine, Michael J. Davis, Esther Bovay, Tatiana V. Petrova)....Pages 97-129 Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice (Sang Heon Suh, Seon Pyo Hong, Intae Park, Joo-Hye Song, Gou Young Koh)....Pages 131-139 Morphological and Functional Analysis of CNS-Associated Lymphatics (Jasmin Herz, Antoine Louveau, Sandro Da Mesquita, Jonathan Kipnis)....Pages 141-151 Morphological Analysis of Schlemm’s Canal in Mice (Benjamin R. Thomson, Susan E. Quaggin)....Pages 153-160 Imaging Lymphatics in Mouse Lungs (Peter Baluk, Donald M. McDonald)....Pages 161-180 Imaging of Endothelial Cell Dynamic Behavior in Zebrafish (Baptiste Coxam, Holger Gerhardt)....Pages 181-195 Visualization and Measurement of Lymphatic Function In Vivo (Samia B. Bachmann, Michael Detmar, Steven T. Proulx)....Pages 197-211 Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells (Daniel T. Sweet, Joshua D. Hall, John Welsh, Mark L. Kahn, Juan M. Jiménez)....Pages 213-227 Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo (Jorge A. Castorena-Gonzalez, Joshua P. Scallan, Michael J. Davis)....Pages 229-248 Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior (Jocelynda Salvador, George E. Davis)....Pages 249-259 Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis (Witold W. Kilarski, Esra Güç, Melody A. Swartz)....Pages 261-277 Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro (Anita Rogic, Francois Auger, Mihaela Skobe)....Pages 279-290 Stimulation and Inhibition of Lymphangiogenesis Via Adeno-Associated Viral Gene Delivery (Sinem Karaman, Harri Nurmi, Salli Antila, Kari Alitalo)....Pages 291-300 Isolation and Characterization of Mouse Organ-Specific Endothelial Transcriptomes (Shahin Rafii, Brisa Palikuqi)....Pages 301-308 Single-Cell mRNA Sequencing of the Mouse Brain Vasculature (Michael Vanlandewijck, Christer Betsholtz)....Pages 309-324 Metabolic Analysis of Lymphatic Endothelial Cells (Pengchun Yu, Tiago C. Alves, Richard G. Kibbey, Michael Simons)....Pages 325-334 Characterizing Epigenetic Changes in Endothelial Cells (Matthew T. Menendez, Courtney T. Griffin)....Pages 335-344 Back Matter ....Pages 345-345 "This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field." -- Prové de l'editor