Neuronal Cell Death : Methods and Protocols

This volume represents a valuable and readily reproducible collection of established and emerging techniques for neuronal cell death research. Conveniently divided into four parts, sections cover a series of techniques for the molecular, structural, functional and genomic characterization of dying neurons, a number of protocols that are of primary interest in neuropathology and in experimental neuropathology, a series of gene engineering techniques to obtain and manipulate neuronal stem cells and progenitors, to prepare HSV-1 vectors for the gene therapy, and to CNS transplantation of bone marrow stem cells, and finally, some very interesting protocols for the study of cell death in non-mammalian models. Written in the successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, __Neuronal Cell Death: Methods and Protocols__ seeks to serve a large audience of scientists that are currently active in the field or are willing to enter such an exciting and still expanding area of neurobiology. Neuronal cell death : an overview of its different forms in central and peripheral neurons -- Laura Lossi, Claudia Castagna, and Adalberto Merighi Cell volume regulation monitored with combined epifluorescence and digital holographic microscopy -- Nicolas Pavillon and Pierre Marquet Flow cytometric analysis of DNA synthesis and apoptosis in central nervous system using fresh cell nuclei -- Noelia López-Sánchez and José M. Frade Nuclear signs of pre-neurodegeneration -- Fernando C. Baltanás [and four others] Multi-parametric O2 imaging in three-dimensional neural cell models with the phosphorescent probes -- Ruslan I. Dimitriev and Dmitri B. Papkovsky Calcium imaging in neuron cell death -- María Calvo, Carlos Villalobos, and Lucía Núñez Monitoring mitochondrial membranes permeability in live neurons and mitochondrial swelling through electron microscopy analysis -- Macarena S. Arrázola and Nibaldo C. Inestrosa Real-time visualization of caspase-3 activation by fluorescence resonance energy transfer (FRET) -- Silvia Alasia [and three others] Design and cloning of short hairpin RNAs (shRNAs) into a lentiviral silencing vector to study the function of selected proteins in neuronal apoptosis -- Nadia Canu Genomic analysis using affymetrix standard microarray GeneChips (169 format) in degenerate murine retina -- Sook Hyun Chung , Weiyong Shen , and Mark Gillies Genomic analysis of transcriptional changes underlying neuronal apoptosis -- Sebastiano Cavallaro High-throughput cell death assays -- Matthew E. Pamenter and Gabriel G. Haddad Staining of dead neurons by the Golgi method in autopsy material -- Stavros J. Baloyannis Image analysis algorithms for immunohistochemical assessment of cell death -- Stan Krajewski [and five others] In vitro oxygen-glucose deprivation to study ischemic cell death -- Carla I. Tasca, Tharine Dal-Cim, and Helena Cimarosti Laser microbeam targeting of single nerve axons in cell culture -- Nicholas Hyun, Linda Z. Shi , and Michael W. Berns Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy -- Eduardo M. Normando [and five others] Targeted toxicants to dopaminergic neuronal cell death -- Huajun Jin [and five others] Stem cells, neural progenitors, and engineered stem cells -- Raj R. Rao and Shilpa Iyer Herpes simplex virus type 1 (HSV-1)-derived recombinant vectors for gene transfer and gene therapy -- Peggy Marconi , Cornel Fraefel, and Alberto L. Epstein Herpes simplex virus type 1 (HSV-1)-derived amplicon vectors for gene transfer and gene therapy -- Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein Bone marrow transplantation for research and regenerative therapies in the central nervous system -- David Díaz, José Ramón Alonso, and Eduardo Weruaga Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA) -- Antón Barreiro-Iglesias and Michael I. Shifman Generation of Zebrafish models by CRISPR/Cas9 genome editing -- Alexander Hruscha and Bettina Schmid In vivo assessment of neuronal cell death in Drosophila -- Pierre Dourlen Drosophila model for studying phagocytosis following neuronal cell death -- Boris Shklyar, Flonia Levy-Adam, and Estee Kurant. Front Matter....Pages i-xiv Front Matter....Pages 1-18 Front Matter....Pages 19-19 Front Matter....Pages 21-32 Front Matter....Pages 33-42 Front Matter....Pages 43-54 Back Matter....Pages 55-71 ....Pages 73-85