Recombinant DNA Technology

Recombinant DNA Technology focuses on the current state of knowledge on recombinant DNA (rDNA) technology and its applications. The book provides comprehensive information on the principles and concepts of rDNA technology or genetic engineering, protein expression of cloned genes, polymerase chain reaction (PCR) amplification of DNA, restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), DNA fingerprinting, and the most recent siRNA technology. It is useful for post-graduate students and teachers in the areas of molecular biology, biotechnology, genetics, microbiology, life sciences, pharmacy, agriculture, and basic medical sciences. Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences. Key Comprehensive analysis of the DNA structure and the process of DNA replication Extensive analysis of restriction endonucleases, sequencing by Sangers method, and protein production in bacteria Detailed description of PCR, RFLP, AFLP, and DNA fingerprinting Thorough explanation of site-directed mutagenesis and cloning in cosmid vectors In-depth discussion on methods for creating recombinant DNA molecules and construction of DNA libraries Concepts and applications of agarose gel and polyacrylamide gel electrophoresis Recombinant DNA Technology Methods for Creating Recombinant DNA Molecules Properties of Restriction Endonucleases Screening of Recombinant DNA Molecules Construction of DNA Library Sequencing by Sangers Method Protein Production in Bacteria Site-directed Mutagenesis Restriction Fragment Length Polymorphism Polymerase Chain Reaction DNA Fingerprinting RNAi and siRNA Technology Molecular Biology Methods Features of Commonly Used Vectors Isolation and Purification of Plasmid Vectors Cloning in Cosmid Vectors Construction of Genomic DNA Libraries in Cosmid Vectors Enzymes Used in Molecular Cloning Agarose Gel and Polyacrylamide Gel Electrophoresis Detection and Extraction of DNA from Gels Revision Questions Bibliography Glossary Colour Plates Index About the Author Annotation Recombinant DNA Technology is focussed on the current state of knowledge on the recombinant DNA technology and its applications. The book will provide comprehensive knowledge on the principles and concepts of recombinant DNA technology or genetic engineering, protein expression of cloned genes, PCR amplification of DNA, RFLP, AFLP and DNA fingerprinting and finally the most recent siRNA technology. It can be used by post-graduate students studying and teachers teaching in the area of Molecular Biology, Biotechnology, Genetics, Microbiology, Life Science, Pharmacy, Agriculture and Basic Medical Sciences