Recombinant DNA Technology

Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences. TABLE OF CONTENTS 6 LIST OF ILLUSTRATIONS 8 LIST OF TABLES 10 PREFACE 12 ABBREVATIONS 14 CHAPTER ONE GENE CLONING: AN OVERVIEW 18 CHAPTER Two GENE LIBRARIES CONSTRUCTION AND SCREENING 36 CHAPTER THREE CONCEPTS AND ApPROACHES IN PLANT GENETIC TRANSFORMATION 42 CHAPTER FOUR MARKER GENES : TOOLS FOR TRACKING PLANT TRANSFORMATION EVENTS 58 CHAPTER FIVE TECHNIQUES IN MOLECULAR BIOLOGY 68 CHAPTER SIX GENOME EDITING ApPROACHES 84 CHAPTER SEVEN ApPLICATION OF RECOMBINANT DNA TECHNOLOGY 92 ApPENDICES 100 GLOSSARY 102 BIBLIOGRAPHY 106 INDEX 118